Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 May 1;13(5):1030-1034.
eCollection 2020.

Hypoxia promotes proliferation of pituitary adenomas by HIF-1α/ALKBH5 signaling in vitro

Yuan Qian  1   2 , Chao Zhang  3 , Wei Wang  3   4 , Di Lu  5 , Junjun Li  3 , Liyan Li  6 , Yao Li  3 , Yisheng Qiao  3 , Hao Song  3 , Xingli Deng  3
Affiliations

Hypoxia promotes proliferation of pituitary adenomas by HIF-1α/ALKBH5 signaling in vitro

Yuan Qian et al. Int J Clin Exp Pathol. .

Abstract

Hypoxia is a common phenomenon in pituitary adenomas (PAs). The role and mechanism of hypoxia in the PAs remains elusive. This work aimed to explore the effect of hypoxia on PAs in vitro. PA cells GT1-1 were cultured and treated under hypoxic condition. Cell proliferation assay showed the proliferation of PA cells was increased significantly by hypoxia treatment, with a peak at 12 hours. qPCR and western blot indicated that the expression of HIF-1α, ALKBH5, and Nanog were elevated by hypoxia stimuli. In conclusion, our funding demonstrated that hypoxia could increase Nanog expression through HIF-1α/ALKBH5 signaling, thereby promoting the proliferation of PA cells.

Keywords: ALKBH5; HIF-1α; Nanog; Pituitary adenoma; hypoxia.

PubMed Disclaimer

Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Effect of hypoxia on the proliferation of GT1-1 cells in vitro. *P<0.05, ***P<0.001.
Figure 2
Figure 2
Effects of hypoxia on the expression of HIF-1α, ALKBH5, and Nanog mRNA in GT1-1 cells. *P<0.05, ***P<0.001.
Figure 3
Figure 3
Effects of hypoxia on the expression of HIF-1α, ALKBH5, and Nanog protein in GT1-1 cells. *P<0.05, ***P<0.001.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Ostrom QT, Gittleman H, Truitt G, Boscia A, Kruchko C, Barnholtz-Sloan JS. CBTRUS statistical report: primary brain and other central nervous system tumors diagnosed in the United States in 2011-2015. Neuro Oncol. 2018;20(Suppl 4):iv1–iv86. - PMC - PubMed
    1. Kristof RA, Aliashkevich AF, Hans V, Haun D, Meyer B, Thees C, Schramm J. The regional oxygen saturation of pituitary adenomas is lower than that of the pituitary gland: microspectrophotometric study with potential clinical implications. Neurosurgery. 2003;53:880–885. - PubMed
    1. He W, Huang L, Shen X, Yang Y, Wang D, Yang Y, Zhu X. Relationship between RSUME and HIF-1α/VEGF-A with invasion of pituitary adenoma. Gene. 2017;603:54–60. - PubMed
    1. Zhang C, Qiang Q, Jiang Y, Hu L, Ding X, Lu Y, Hu G. Effects of hypoxia inducible factor-1α on apoptotic inhibition and glucocorticoid receptor downregulation by dexamethasone in AtT-20 cells. BMC Endocr Disord. 2015;15:24. - PMC - PubMed
    1. Yoshida D, Teramoto A. Elevated cell invasion is induced by hypoxia in a human pituitary adenoma cell line. Cell Adh Migr. 2007;1:43–51. - PMC - PubMed

LinkOut - more resources