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. 2020 May 1;13(5):1045-1052.
eCollection 2020.

Differential expression profiles of circRNAs in human prostate cancer based on chip and bioinformatic analysis

Shengyang Ge  1 , Chuanyu Sun  1 , Qingfeng Hu  1 , Yijun Guo  2 , Guowei Xia  1 , Yuanyuan Mi  3 , Lijie Zhu  3
Affiliations

Differential expression profiles of circRNAs in human prostate cancer based on chip and bioinformatic analysis

Shengyang Ge et al. Int J Clin Exp Pathol. .

Abstract

Background: Increasing evidence suggests that circRNAs are involved in the pathogenesis of multiple kinds of cancer. Nevertheless, the differential expression of circRNAs in prostate cancer (PCA) is rarely reported.

Material/method: In our present analyses, circRNAs expression profiles were identified in PCA, based on 5 pairs of PCA and matched non-PCA tissues using circRNA chips.

Results: A number of 749 differential circRNAs were expressed between PCA tumor and paracancerous tissues (Fold Change, FC ≥ 2.0 and P < 0.05): 261 were upregulated, whereas 487 were downregulated in PCA tissues. Gene ontology and KEGG pathway analyses indicated that many of the circRNAs are related to carcinogenesis. Circ_0033074 and circ_0016064 both showed changes of maximum magnitude among differentially expressed circRNAs.

Conclusions: Our study detected a relative comprehensive differential map of circRNAs in PCA, which may become novel biomarkers for diagnosis, treatment and follow-up in the future.

Keywords: Prostate cancer; circRNA; expression; microarray.

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Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Hierarchical clustering, volcano plots, and scatter plots displayed the differentially expressed circRNAs in PCA tissues compared to paracancerous tissues. A. Hierarchical clustering: numbers were the samples used for the microarray assay. T: PCA tissues, N: paracancerous tissues. B. Differentially expressed circRNAs are shown as volcano plots. The blue and red parts indicate (FC more than 2 folds) downregulated and upregulated expression circRNAs in PCA tissues, respectively (P < 0.05). C. Differentially expressed circRNAs shown by scatter plots. The green and red parts indicate (FC more than 2 folds) downregulated and upregulated expression circRNAs in PCA tissues, respectively (P < 0.05).
Figure 2
Figure 2
GO and KEGG pathway analysis of differentially expressed circRNAs. A. GO enrichment terms of top 30 classes. B. KEGG pathway enrichment terms of top 30 classes.
See this image and copyright information in PMC

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