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[Preprint]. 2020 Apr 18:2020.04.13.20063784.
doi: 10.1101/2020.04.13.20063784.

Comparative dynamic aerosol efficiencies of three emergent coronaviruses and the unusual persistence of SARS-CoV-2 in aerosol suspensions

A C Fears  1 , W B Klimstra  2 , P Duprex  2 , A Hartman  1   2   3   4   5 , S C Weaver  3 , K C Plante  3 , D Mirchandani  3 , J A Plante  3 , P V Aguilar  3 , D Fernández  3 , A Nalca  4 , A Totura  4 , D Dyer  4 , B Kearney  4 , M Lackemeyer  5 , J K Bohannon  5 , R Johnson  5 , R F Garry  1 , D S Reed #  2 , C J Roy #  1
Affiliations

Comparative dynamic aerosol efficiencies of three emergent coronaviruses and the unusual persistence of SARS-CoV-2 in aerosol suspensions

A C Fears et al. medRxiv. .

Update in

  • Persistence of Severe Acute Respiratory Syndrome Coronavirus 2 in Aerosol Suspensions.
    Fears AC, Klimstra WB, Duprex P, Hartman A, Weaver SC, Plante KS, Mirchandani D, Plante JA, Aguilar PV, Fernández D, Nalca A, Totura A, Dyer D, Kearney B, Lackemeyer M, Bohannon JK, Johnson R, Garry RF, Reed DS, Roy CJ. Fears AC, et al. Emerg Infect Dis. 2020 Sep;26(9):2168-71. doi: 10.3201/eid2609.201806. Epub 2020 Jun 22. Emerg Infect Dis. 2020. PMID: 32568661 Free PMC article.
No abstract available

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Figures

Figure 1.
Figure 1.. Aerosol efficiency of MERS-CoV, SARS-CoV and SARS-CoV-2 at different sites.
Graph shows the spray factor (Fs; the ratio of the nebulizer concentration to the aerosol concentration) for MERS-CoV (red symbols), SARS-CoV (blue symbols), and SARS-CoV-2 (green symbols). Aerosols were performed at four different sites (S1 = Tulane; S2 = NIH-IRF; S3 = USAMRIID S4 = Pittsburgh) and with different nebulizers (C3 = Collison 3-jet; C6 = Collison 6-jet; AS = Aerogen Solo).
Figure 2.
Figure 2.. Decay curves of SARS-CoV-2 in aerosol suspension.
A. Aerosol concentration of infectious SARS-CoV-2 as measured by plaque assay found in impinger samples collected at five differing timepoints of increased aging in aerosol suspension, B. Corresponding aerosol concentration of SARS-CoV-2 in time-matched impinger samples as a function of viral genome copies as measured by qPCR. Both timepoint virus estimates were graphed and nonlinear least-squares regression analysis single-order decay with no outlier detection was performed, resulting in a poor curve fit by either method of viral quantitation resulting from number and lack of iterative samples in this analysis.
Figure 3.
Figure 3.. Electron microscopy images of SARS-CoV-2 in aerosol suspension at various timepoints.
A, B: from viral stock prior to aerosolization; C, D: from 10 minute aerosol suspension; E, F: from 16 hour aerosol suspension.
See this image and copyright information in PMC

References

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