Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep;46(9):1690-1701.
doi: 10.1111/jog.14341. Epub 2020 Jun 8.

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Jun Tao  1 , Lin-Zhen Xia  1 , Jiao-Jiao Chen  1 , Jun-Fa Zeng  1 , Jun Meng  2 , ShiYuan Wu  3 , Zuo Wang  1
Affiliations

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Jun Tao et al. J Obstet Gynaecol Res. 2020 Sep.

Abstract

Aim: This study aimed to investigate the effect of high glucose (HG) level on the proliferation, migration and invasion of trophoblasts and determine the role of placental growth factor (PLGF) in the process.

Methods: HTR8-S/Vneo was treated with different concentrations of d-glucose (0, 10, 15, 20, 25 and 30 μM) at different times (0, 6, 12 and 24 h). qRT-PCR and Western blot analyses were used to measure PLGF expression. The protein level of PLGF was measured by immunofluorescence. Cell proliferation was assessed with CCK-8 analysis. Wound healing and transwell assays were used to evaluate cell migration and invasion. Intercellular ROS was detected with DCFH-DA.

Results: After d-glucose treatment, the viability decreased in 25 and 30 μM groups. The HG group (25 μM) showed inhibited cell migration and invasion ability. The mRNA and protein levels of PLGF decreased under HG condition. Elevated ROS production was also detected in the HG group. Knocked-down PLGF expression enhanced increased ROS production and decreased cell migration and invasion, which reverted to the original levels after PLGF was overexpressed.

Conclusion: High glucose treatment inhibited HTR8-S/Vneo viability, migration and invasion by downregulating PLGF expression.

Keywords: HTR8-S/Vneo; gestational diabetes mellitus; placental growth factor; pre-eclampsia; reactive oxygen species.

PubMed Disclaimer

References

Reference

    1. Jim B, Karumanchi SA. Preeclampsia: Pathogenesis, prevention, and Long-term complications. Semin Nephrol 2017; 37: 386-397.
    1. Yang X, Meng T. MicroRNA-431 affects trophoblast migration and invasion by targeting ZEB1 in preeclampsia. Gene 2019; 683: 225-232.
    1. Red-Horse K, Zhou Y, Genbacev O et al. Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface. J Clin Invest 2004; 114: 744-754.
    1. Staun-Ram E, Shalev E. Human trophoblast function during the implantation process. Reprod Biol Endocrinol 2005; 3: 56.
    1. Hastie R, Brownfoot FC, Pritchard N et al. EGFR (epidermal growth factor receptor) signaling and the mitochondria regulate sFlt-1 (soluble FMS-like tyrosine kinase-1) secretion. Hypertension 2019; 73: 659-670.

LinkOut - more resources