Dietary Supplementation of Postbiotics Mitigates Adverse Impacts of Heat Stress on Antioxidant Enzyme Activity, Total Antioxidant, Lipid Peroxidation, Physiological Stress Indicators, Lipid Profile and Meat Quality in Broilers

Abstract

The purpose of this work was to evaluate the impacts of feeding different postbiotics on oxidative stress markers, physiological stress indicators, lipid profile and meat quality in heat-stressed broilers. A total of 252 male Cobb 500 (22-day-old) were fed with 1 of 6 diets: A basal diet without any supplementation as negative control (NC); basal diet + 0.02% oxytetracycline served as positive control (PC); basal diet + 0.02% ascorbic acid (AA); or the basal diet diet + 0.3% of RI11, RS5 or UL4 postbiotics. Postbiotics supplementation, especially RI11 increased plasma activity of total-antioxidant capacity (T-AOC), catalase (CAT) and glutathione (GSH), and decreased alpha-1-acid-glycoprotein (α1-AGP) and ceruloplasmin (CPN) compared to NC and PC groups. Meat malondialdehyde (MDA) was lower in the postbiotic groups than the NC, PC and AA groups. Plasma corticosterone, heat shock protein70 (HSP70) and high density lipoprotein (HDL) were not affected by dietary treatments. Postbiotics decreased plasma cholesterol concentration compared to other groups, and plasma triglyceride and very low density lipoprotein (VLDL) compared to the NC group. Postbiotics increased breast meat pH, and decreased shear force and lightness (L*) compared to NC and PC groups. The drip loss, cooking loss and yellowness (b*) were lower in postbiotics groups compared to other groups. In conclusion, postbiotics particularly RI11 could be used as an alternative to antibiotics and natural sources of antioxidants for heat-stressed broilers.

Keywords: acute phase proteins; antibiotic; antioxidant enzyme activity; broilers; heat shock protein 70; heat stress; lipid peroxidation; lipid profile; meat quality; postbiotic.

Figure 1

Effect of feeding different postbiotics on plasma acute phase proteins (α1-AGP and CPN), and HSP70 concentrations in heat-stressed broiler chickens. a,b,c,d standard error bars sharing different letters are significantly different (p < 0.05). Data are shown as means and standard error (n = 12). α1-AGP: alpha-1-acid-glycoprotein, HSP70: heat shock protein 70, CPN: ceruloplasmin. Diets: Negative control (NC): basal diet without any supplementation, positive control (PC): basal diet + 0.02% oxytetracycline (w/w), AA: basal diet + 0.02% ascorbic acid (w/w), RI11: basal diet + 0.3% postbiotic RI11 (v/w), RS5: basal diet + 0.3% postbiotic RS5 (v/w), UL4: basal diet + 0.3% postbiotic UL4 (v/w).

Figure 2

Effect of feeding different postbiotics on plasma corticosterone concentration in heat-stressed broiler chickens. Data represent means and standard error (n = 12). Diets: Negative control (NC): basal diet without any supplementation, Positive control (PC): basal diet + 0.02% oxytetracycline (w/w), AA: basal diet + 0.02% ascorbic acid (w/w), RI11: basal diet + 0.3% postbiotic RI11 (v/w), RS5: basal diet + 0.3% postbiotic RS5 (v/w), UL4: basal diet + 0.3% postbiotic UL4 (v/w).