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. 2020 Jun 8;12(6):624.
doi: 10.3390/v12060624.

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Boris Pastorino  1 , Franck Touret  1 , Magali Gilles  1 , Lea Luciani  1 , Xavier de Lamballerie  1 , Remi N Charrel  1
Affiliations

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Boris Pastorino et al. Viruses. .

Abstract

Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.

Keywords: COVID-19; SARS-CoV-2; coronavirus; extraction buffer; inactivation; lysis buffer.

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Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Cascella M., Rajnik M., Cuomo A., Dulebohn S.C., Di Napoli R. StatPearls. StatPearls Publishing; Treasure Island, FL, USA: 2020. Features, Evaluation and Treatment Coronavirus (COVID-19) - PubMed
    1. Otter J.A., Donskey C., Yezli S., Douthwaite S., Goldenberg S.D., Weber D.J. Transmission of SARS and MERS coronaviruses and influenza virus in healthcare settings: The possible role of dry surface contamination. J. Hosp. Infect. 2016;92:235–250. doi: 10.1016/j.jhin.2015.08.027. - DOI - PMC - PubMed
    1. Xiao S., Li Y., Wong T., Hui D.S.C. Role of fomites in SARS transmission during the largest hospital outbreak in Hong Kong. PLoS ONE. 2017;12:e0181558. doi: 10.1371/journal.pone.0181558. - DOI - PMC - PubMed
    1. Ong S.W.X., Tan Y.K., Chia P.Y., Lee T.H., Ng O.T., Wong M.S.Y., Marimuthu K. Air, Surface Environmental, and Personal Protective Equipment Contamination by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) From a Symptomatic Patient. JAMA. 2020 doi: 10.1001/jama.2020.3227. - DOI - PMC - PubMed
    1. Laboratory Biosafety Guidance Related to the Novel Coronavirus (2019-nCoV)-Interim Guidance. [(accessed on 8 June 2020)];2020 Available online: https://www.who.int/publications/i/item/laboratory-biosafety-guidance-re...

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