Axon Degeneration: Which Method to Choose?

Abstract

Axons are diverse. They have different lengths, different branching patterns, and different biological roles. Methods to study axon degeneration are also diverse. The result is a bewildering range of experimental systems in which to study mechanisms of axon degeneration, and it is difficult to extrapolate from one neuron type and one method to another. The purpose of this chapter is to help readers to do this and to choose the methods most appropriate for answering their particular research question.

Keywords: Axonal transport; Drosophila; Explant culture; Nerve lesion; Primary neuronal culture.