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. 2020:2152:437-443.
doi: 10.1007/978-1-0716-0640-7_32.

Measuring the Kinase Activity of GCKIII Proteins In Vitro

Juan Zalvide  1 , Cristina Almengló  2   3 , Sara Vázquez  2 , Mar García-Colomer  2 , Miriam Sartages  2 , Celia M Pombo  4
Affiliations

Measuring the Kinase Activity of GCKIII Proteins In Vitro

Juan Zalvide et al. Methods Mol Biol. 2020.

Abstract

One of the CCM genes, CCM3/PDCD10, binds to the protein kinase family GCKIII, which comprises MST3/STK24, SOK1/STK25, and MST4/STK26. These proteins have been shown to have the same effect as CCM3, both in endothelial cells and in animal models such as zebrafish and are most likely involved in CCM pathogenesis. We describe here an in vitro kinase assay of GCKIII proteins which can be used to study their regulation in endothelial and other cells under different circumstances.

Keywords: CCM3; Cellular stress; GCKIII.

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