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. 2020 Aug 5;154(3):342-352.
doi: 10.1093/ajcp/aqaa057.

CALIPER Hematology Reference Standards (II)

Victoria Higgins  1   2 , Houman Tahmasebi  1 , Mary Kathryn Bohn  1   2 , Alexandra Hall  1 , Khosrow Adeli  1   2
Affiliations

CALIPER Hematology Reference Standards (II)

Victoria Higgins et al. Am J Clin Pathol. .

Abstract

Objectives: The objective of this study was to establish comprehensive age- and sex-specific reference intervals for hematologic parameters in the CALIPER cohort of healthy children and adolescents.

Methods: A total of 536 healthy children and adolescents (birth to 21 years) were recruited with informed consent, and whole blood samples were analyzed for 27 hematologic parameters on the Beckman Coulter DxH 520 system. Age- and sex-specific pediatric reference standards were established. Reference values obtained on the DxH 520 were also compared with data obtained on a larger laboratory-based instrument (DxH 900).

Results: Most hematologic parameters showed significant age- and/or sex-specific changes during growth and development. Of the 27 hematologic parameters, all except four (mean corpuscular hemoglobin concentration, basophil percentage, low hemoglobin density, immature cell percentage) required age partitioning, and eight required sex partitioning.

Conclusions: This study establishes a robust pediatric hematology reference database that will assist in more accurate test result interpretation. Our data clearly demonstrate significant variation in hematologic parameter concentrations in children and adolescents, necessitating the use of pediatric-specific reference standards.

Keywords: Beckman Coulter; CALIPER; Hematology; Pediatric; Reference intervals.

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Figures

Figure 1
Figure 1
Age- and sex-specific scatterplots of pediatric reference values for RBC count (A), hemoglobin (B), hematocrit (C), mean corpuscular volume (D), mean corpuscular hemoglobin (E), and mean corpuscular hemoglobin concentration (F). Males are shown in blue and females are shown in pink.
Figure 2
Figure 2
Age- and sex-specific scatterplots of pediatric reference values for RBC distribution width (A), RBC distribution width–standard deviation (B), platelet count (C), and mean platelet volume (D). Males are shown in blue and females are shown in pink.
Figure 3
Figure 3
Age- and sex-specific scatterplots of pediatric reference values for WBC count (A), lymphocyte percentage (B), monocyte percentage (C), neutrophil percentage (D), eosinophil percentage (E), and basophil percentage (F). Males are shown in blue and females are shown in pink.
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References

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