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. 2020 May 26:11:906.
doi: 10.3389/fmicb.2020.00906. eCollection 2020.

Patellariopsidaceae Fam. Nov. With Sexual-Asexual Connection and a New Host Record for Cheirospora botryospora (Vibrisseaceae, Ascomycota)

Anuruddha Karunarathna  1   2   3   4   5 , Derek Peršoh  6 , Anusha H Ekanayaka  4   5 , Ruvishika S Jayawardena  5 , K W Thilini Chethana  5 , Ishani D Goonasekara  3   4   5 , Ratchadawan Cheewangkoon  2   7 , Erio Camporesi  8   9 , Kevin D Hyde  3   4   5 , Saisamorn Lumyong  1   7   10 , Samantha C Karunarathna  1   3   4
Affiliations

Patellariopsidaceae Fam. Nov. With Sexual-Asexual Connection and a New Host Record for Cheirospora botryospora (Vibrisseaceae, Ascomycota)

Anuruddha Karunarathna et al. Front Microbiol. .

Abstract

Helotiales is a polyphyletic order of Ascomycetes. The paucity of relevant molecular data and unclear connections of sexual and asexual morphs present challenges in resolving taxa within this order. In the present study, Patellariopsidaceae fam. nov., the asexual morph of Patellariopsis atrovinosa, and a new record of Cheirospora botryospora (Vibrisseaceae) on Fagus sylvatica (Fagaceae) from Italy are discussed based on morphology and molecular phylogeny. Phylogenetic analyses based on a combined sequence dataset of LSU and ITS were used to infer the phylogenetic relationships within the Helotiales. The results of this research provide a solid base to the taxonomy and phylogeny of Helotiales.

Keywords: Ascomycetes; Cheirospora botryospora; Leotiomycetes; Pezizomycotina; sporodochium.

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Figures

FIGURE 1
FIGURE 1
RAxML tree based on a combined dataset of LSU and ITS partial sequence data. Bootstrap support values for maximum likelihood equal to or higher than 70%, maximum parsimony equal to or higher than 70%, and Bayesian posterior probabilities equal to or greater than 0.90 are displayed on the nodes, respectively. Newly generated sequences are indicated in white. The tree is rooted to Lambertella seditiosa and Rutstroemia longipes.
FIGURE 2
FIGURE 2
Asexual morph of Patellariopsis atrovinosa (MFLU 16-2950). (A,B) Appearance of sporodochium on host substrate. (C) Longitudinal section of sporodochium. (D) Conidiophore attached to the host. (E–G) Various stages of conidiogenesis. (H–J) Conidia. (K) Germinated conidium. (L,M) Culture characteristics on PDA (L = from above, M = from below). Scale bars: C = 50 μm; D,E = 20 μm; F–K = 10 μm.
FIGURE 3
FIGURE 3
Asexual morph of C. botryospora (MFLU 15-2612). (A,B) Appearance of sporodochium on host substrate. (C) Longitudinal section of sporodochium. (D,E) Conidia attached to the conidiophores. (F–J) Conidia. (K) Conidia surrounded by mucilaginous sheath, stained with Indian ink. (L) Germinated conidium. (M,N) Culture characteristics on PDA (M = From above, N = From below). Scale bars: C = 20 μm; D,E = 50 μm; F–J = 10 μm.
See this image and copyright information in PMC

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