Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Meta-Analysis
. 2020 Nov;50(11):1757-1769.
doi: 10.1002/eji.201948489. Epub 2020 Jun 23.

Transcriptional meta-analysis of regulatory B cells

Florian Dubois  1   2 , Sophie Limou  1   3 , Mélanie Chesneau  1   2 , Nicolas Degauque  1   2 , Sophie Brouard  1   2   4 , Richard Danger  1   2
Affiliations
Free article
Meta-Analysis

Transcriptional meta-analysis of regulatory B cells

Florian Dubois et al. Eur J Immunol. 2020 Nov.
Free article

Abstract

Regulatory B cells (Bregs) have the ability to regulate inflammation in various pathological situations, making them key players in immune regulation. Several mechanisms have been described and we recently identified a GZMB expressing Breg population in kidney transplanted patients who tolerate a kidney graft. To further investigate their biology and mechanisms, we conducted a transcriptomic analysis by RNAseq of these cells and we performed the first weighted meta-analysis of publicly available transcriptomic data from published Breg studies both in humans and mice. We identified two distinct and unique transcriptional signatures of 126 and 93 genes, respectively, associated with these Bregs. While we highlighted genes coding for proteins with potent involvement in regulatory functions, proliferation, and coding for transcription factors, the comparison between humans and mice did not allow identifying a common pattern. Thus, our results suggest distinct species-restricted Breg transcriptional signatures in humans and mice.

Keywords: B cells; Bregs; gene expression; granzyme B; meta-analysis.

PubMed Disclaimer

References

    1. Wasik, M., Nazimek, K. and Bryniarski, K., Regulatory B cell phenotype and mechanism of action: the impact of stimulating conditions. Microbiol. Immunol. 2018. 62: 485-496.
    1. Fillatreau, S., Sweenie, C. H., McGeachy, M. J., Gray, D. and Anderton, S. M., B cells regulate autoimmunity by provision of IL-10. Nat. Immunol. 2002. 3: 944-950.
    1. Mauri, C., Gray, D., Mushtaq, N. and Londei, M., Prevention of arthritis by interleukin 10-producing B cells. J. Exp. Med. 2003. 197: 489-501.
    1. Mizoguchi, A., Mizoguchi, E., Takedatsu, H., Blumberg, R. S. and Bhan, A. K., Chronic intestinal inflammatory condition generates IL-10-producing regulatory B cell subset characterized by CD1d upregulation. Immunity 2002. 16: 219-230.
    1. Iwata, Y., Matsushita, T., Horikawa, M., Dilillo, D. J., Yanaba, K., Venturi, G. M., Szabolcs, P. M., et al., Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. Blood 2011. 117: 530-541.

Publication types