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. 2020 Nov;20(11):1231-1232.
doi: 10.1016/S1473-3099(20)30362-5. Epub 2020 Apr 28.

Pooling of samples for testing for SARS-CoV-2 in asymptomatic people

Stefan Lohse  1 , Thorsten Pfuhl  1 , Barbara Berkó-Göttel  1 , Jürgen Rissland  1 , Tobias Geißler  2 , Barbara Gärtner  3 , Sören L Becker  3 , Sophie Schneitler  3 , Sigrun Smola  4
Affiliations

Pooling of samples for testing for SARS-CoV-2 in asymptomatic people

Stefan Lohse et al. Lancet Infect Dis. 2020 Nov.
No abstract available

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Figures

Figure
Figure
Ct values of single versus pooled samples Absolute Ct values of positive pools (13 of 164 tested pools) in relation to pool size and corresponding Ct values of individual positive samples for the E-gene assay (A) and the S-gene assay (B). Absolute Ct values were below 30 for all pool sizes. Three positive individual samples with Ct values greater than 30 were spiked into negative pools of 30 samples and tested with E-gene (C) and S-gene (D) assays. We hypothesise that the lower Ct values of pools than of single samples were because of the carrier effect of the higher RNA content in pools. Connecting lines show positive single samples and their corresponding pools. Ct=cycle threshold. E-gene=envelope protein gene. S-gene=spike protein gene.
See this image and copyright information in PMC

Comment in

  • Challenges and issues of SARS-CoV-2 pool testing.
    Lee J, Kim SY, Sung H, Lee SW, Lee H, Roh KH, Yoo CK, Hong KH. Lee J, et al. Lancet Infect Dis. 2020 Nov;20(11):1232-1233. doi: 10.1016/S1473-3099(20)30516-8. Epub 2020 Jul 14. Lancet Infect Dis. 2020. PMID: 32679080 Free PMC article. No abstract available.
  • Challenges and issues of SARS-CoV-2 pool testing.
    Mishra B, Behera B, Mohanty M, Ravindra A, Ranjan J. Mishra B, et al. Lancet Infect Dis. 2020 Nov;20(11):1233. doi: 10.1016/S1473-3099(20)30463-1. Epub 2020 Jul 14. Lancet Infect Dis. 2020. PMID: 32679083 Free PMC article. No abstract available.
  • Challenges and issues of SARS-CoV-2 pool testing - Authors' reply.
    Lohse S, Pfuhl T, Berkó-Göttel B, Gärtner B, Becker SL, Schneitler S, Smola S. Lohse S, et al. Lancet Infect Dis. 2020 Nov;20(11):1234-1235. doi: 10.1016/S1473-3099(20)30455-2. Epub 2020 Jul 14. Lancet Infect Dis. 2020. PMID: 32679086 Free PMC article. No abstract available.
  • Challenges and issues of SARS-CoV-2 pool testing.
    Eberhardt JN, Breuckmann NP, Eberhardt CS. Eberhardt JN, et al. Lancet Infect Dis. 2020 Nov;20(11):1233-1234. doi: 10.1016/S1473-3099(20)30467-9. Epub 2020 Jul 14. Lancet Infect Dis. 2020. PMID: 32679088 Free PMC article. No abstract available.

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