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. 2020 Jul;20(1):543-549.
doi: 10.3892/etm.2020.8728. Epub 2020 May 7.

Virological investigation of genetic variation of enterovirus type 71 in hand, foot and mouth disease

Yang Wang  1 , Yi Li  1 , Yuling Yang  2 , Chuanmei Peng  1 , Xiaoye Fu  1 , Xin Gu  1 , Hui Gao  1
Affiliations

Virological investigation of genetic variation of enterovirus type 71 in hand, foot and mouth disease

Yang Wang et al. Exp Ther Med. 2020 Jul.

Abstract

The aim of the present study was to analyze the sequence of the VP1 gene in enterovirus 71 (EV71) isolates and to explore their genetic evolution, so as to provide a scientific basis for the clinical prevention and treatment of hand, foot and mouth disease. The fecal samples of 590 patients with suspected hand, foot and mouth disease treated at Yan'an Hospital (Kunming, China) between January 2015 and December 2016 were collected and EV71 nucleic acid was detected by fluorescence PCR. The viral RNA of EV71-positive samples was extracted, the VP1 gene was amplified by PCR and the products were sequenced. The VP1 gene sequence was analyzed using DNAMAN and MEGA (version 4.0) software and homologous modeling was performed using Pymol software. A total of 50 EV71-positive samples were identified and the detection rate was 8.47% (50/590 cases). All of the 50 EV71 strains were of the C4 subtype. The genetic distance between the strains detected in the present study and EV71 strains detected in Beijing, Anhui and Malaysia was 0.01-0.03, while that between the strains detected in the present study and Australian strains was 2.11. Homologous modeling indicated that the amino acid sequence of the VP1 gene of the detected strains had a H144Y mutation. There was no significant genetic variation in the EV71 strain within the 2-year period. In conclusion, the EV71 strains detected in the present study was similar to that detected in Beijing, Anhui and Malaysia but different to that from Australia. A point mutation was present in the amino acid sequence of the VP1 gene.

Keywords: enterovirus 71; foot and mouth disease; hand; homology modeling; phylogenetic tree; viral envelope protein 1.

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Figures

Figure 1
Figure 1
Electrophoresis of the VP1 gene amplification products. Lane 1, DNA marker; lane 2, VP1 amplification product of the positive control EV71; lane 3, VP1 positive amplification product of enterovirus 71.
Figure 2
Figure 2
Example of the enterovirus 71 1 VP1 sequence alignment. Sbjct, subject.
Figure 3
Figure 3
Amino acid evolution tree derived from the enterovirus 71 VP1 gene. •Indicates strains isolated in the present study.
Figure 4
Figure 4
Three-dimensional structure of the enterovirus 71 VP1 amino acid mutation site. The left panel refers to the tertiary structure and right panel refers to the secondary structure.
See this image and copyright information in PMC

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