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. 2020 May 13;12(1):1761135.
doi: 10.1080/20002297.2020.1761135. eCollection 2020.

Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy

Jean-Luc C Mougeot  1 , Micaela F Beckman  1 , Craig B Stevens  1 , Kathryn G Almon  1 , Darla S Morton  1 , Inger Von Bültzingslöwen  2 , Michael T Brennan  1 , Farah Bahrani Mougeot  1
Affiliations

Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy

Jean-Luc C Mougeot et al. J Oral Microbiol. .

Abstract

Background: Oral mucositis (OM) is a common side effect of conditioning therapy implemented before hematopoietic stem cell transplantation (HSCT). The role of oral microbiome in OM is not fully elucidated. Objective: To determine oral microbiome profile changes post-conditioning in HSCT patients who developed moderate OM, or mild to no OM. Design: Patient groups were: Muc0-1 with OM-score = 0-1 (43 paired samples) and Muc2 with WHO OM-score = 2 (36 paired samples). Bacterial DNA was isolated from oral samples (saliva, swabs of buccal mucosa, tongue, and supragingival plaque) at pre-conditioning (T 0 ), post-conditioning mucositis onset (T Muc ), and one-year post-conditioning (T Year ). 16S-rRNA gene next-generation sequencing was used to determine the relative abundance (RA) of >700 oral species. Alpha-diversity, beta-diversity and linear discriminant analyses (LDA) were performed Muc2 versus Muc0-1. Results: Muc2 oral microbiome alpha- and beta-diversity differed between T 0 and T Muc . Muc2 alpha-diversity and Muc0-1 beta-diversity did not differ between T 0 and T Year . T 0 to T Muc LDA scores were significant in Muc2 for Gammaproteobacteria. For Muc2 patients, the average RA decreased for Haemophilus parainfluenza, a species known as mucosal surfaces protector, but increased for Escherichia-Shigella genera. Conclusions: Post-conditioning OM might contribute to long-term oral microbiome changes affecting Gammaproteobacteria, in HSCT patients.

Keywords: Mucosal immunity; bacteria; cancer biology; hematologic neoplasms; hematopoietic stem cell transplantation; transplantation conditioning.

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Conflict of interest statement

The authors report no conflict of interest.

Figures

Figure 1.
Figure 1.
Analytical design for fold changes and changes in beta-diversity of hematological cancer patients undergoing conditioning therapy with and without oral mucositis.
Figure 2.
Figure 2.
LEfSe results of set-all Muc0-1 and Muc2 groups.
Figure 2.
Figure 2.
(Continued).
Figure 3.
Figure 3.
Average fold change (RA-FcD) difference from T0 to TMuc distinguishing Muc0-1 from Muc2 hematological cancer patient groups undergoing conditioning therapy.
See this image and copyright information in PMC

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