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Case Reports
. 2020 Dec;55(12):2354-2356.
doi: 10.1038/s41409-020-0972-8. Epub 2020 Jun 15.

COVID-19 in allogeneic stem cell transplant: high false-negative probability and role of CRISPR and convalescent plasma

Alex Niu #  1 , April McDougal #  2 , Bo Ning  3 , Firas Safa  1 , Alfred Luk  2 , David M Mushatt  2 , Adeem Nachabe  4 , Kevin J Zwezdaryk  5 , James Robinson  6 , Tim Peterson  7 , Francisco Socola  1 , Hana Safah  1 , Tony Hu  3 , Nakhle S Saba  8
Affiliations
Case Reports

COVID-19 in allogeneic stem cell transplant: high false-negative probability and role of CRISPR and convalescent plasma

Alex Niu et al. Bone Marrow Transplant. 2020 Dec.
No abstract available

PubMed Disclaimer

Conflict of interest statement

NSS: Advisory board and speaker bureau for ibrutinib. The other authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1. Diagnostic workup for case 1 and 2.
a Transverse CT scan showing lung infiltrates suspicious for COVID-19 disease for case 1. b CRISPR analysis testing for the presence of the viral genome in the shown sample of case 1. We used genomic RNA extracted from heat-inactivated SARS-CoV-2 (NR-52347, BEI) as positive control, and human RNA isolated from HEK293T cells as negative control. Each experiment was run in triplicate. Values that are 3 SD above the negative control mean were considered positive. The level of fluorescence does not reflect viral load, this is a qualitative test. c CRISPR analysis testing for the presence of the viral genome in the shown sample of case 2 tested as in b.
See this image and copyright information in PMC

References

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