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. 2020 Jul;17(7):689-693.
doi: 10.1038/s41592-020-0858-0. Epub 2020 Jun 15.

Highly specific multiplexed RNA imaging in tissues with split-FISH

Jolene Jie Lin Goh #  1 , Nigel Chou #  1 , Wan Yi Seow  1 , Norbert Ha  1 , Chung Pui Paul Cheng  1 , Yun-Ching Chang  2 , Ziqing Winston Zhao  1   3 , Kok Hao Chen  4
Affiliations

Highly specific multiplexed RNA imaging in tissues with split-FISH

Jolene Jie Lin Goh et al. Nat Methods. 2020 Jul.

Erratum in

Abstract

We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues.

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