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. 2020 Dec;129(6):1486-1496.
doi: 10.1111/jam.14750. Epub 2020 Jul 1.

Technological variables influencing the growth and stability of a silage inoculant based on spray-dried lactic acid bacteria

J E Blajman  1 , G Vinderola  2 , A Cuatrin  3 , M S Lingua  4 , R B Páez  3
Affiliations

Technological variables influencing the growth and stability of a silage inoculant based on spray-dried lactic acid bacteria

J E Blajman et al. J Appl Microbiol. 2020 Dec.

Abstract

Aims: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray-drying in order to enhance survival to dehydration and storage stability.

Methods and results: In house-formulated MRS was a suitable low-cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4°C after spray-drying. The use of whey protein concentrate 35 (WPC)-maltodextrin (M) as matrix, inlet temperature of 145-150°C and air flow rate of 601 l h-1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3·96, 73·68 and 90·36% respectively. Those conditions also showed a decrease of 0·855 log CFU per gram after drying, no loss in viability at 4°C for 6 months and 1 log CFU per gram reduction at 25°C.

Conclusions: Stable and economically feasible dehydrated LAB cultures were obtained using alternative culture media, fermentation under uncontrolled pH and optimizing spray-drying process conditions through the desirability function method.

Significance and impact of the study: Our results can be utilized for efficient production and commercialization of several dry LAB.

Keywords: culture media; fermentation; lactic acid bacteria; optimization; spray-drying.

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References

    1. Adesogan, A.T., Arriola, K.G., Jiang, Y., Oyebade, A., Paula, E.M., Pech-Cervantes, A.A., Romero, J.J., Ferraretto, L.F. et al. (2019) Symposium review: technologies for improving fiber utilization. J Dairy Sci 102, 5726-5755.
    1. Anandharaj, M., Rani, R.P. and Swain, M.R. (2017) Production of high-quality probiotics by fermentation. In Microbial Functional Foods and Nutraceuticals ed. Gupta, V.K., Treichel, H., Shapaval, V.O., de Oliveira, L.A. and Tuohy, M.G., p. 235. Chichester: Wiley & Sons.
    1. Antunes, A.E.C., Cazetto, T.F. and Bolini, H.M.A. (2005) Viability of probiotic microorganisms during storage, postacidification and sensory analysis of fat-free yogurts with added whey protein concentrate. Int J Dairy Technol 58, 169-173.
    1. Barbosa, J., Borges, S. and Teixeira, P. (2015) Influence of sub-lethal stresses on the survival of lactic acid bacteria after spray-drying in orange juice. Food Microbiol 52, 77-83.
    1. Barbosa-Cánovas, G.V., Ortega-Rivas, E., Juliano, P. and Yan, H. (2005) Food Powders: Physical Properties, Processing, and Functionality. New York: Kluwer Academic/Plenum Publishers.