Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun 14;10(6):1164.
doi: 10.3390/nano10061164.

Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

M A Martínez-Rodríguez  1 , E Madla-Cruz  2 , V H Urrutia-Baca  3 , M A de la Garza-Ramos  4 , V A González-González  1   5 , M A Garza-Navarro  1   5
Affiliations

Influence of Polysaccharides' Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

M A Martínez-Rodríguez et al. Nanomaterials (Basel). .

Abstract

In this paper we report on the influence of polysaccharides' molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC). These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw). The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques. The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis. The cytotoxicity of composites was assessed against human gingival fibroblast. Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation. This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO- moieties of CMC. Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.

Keywords: antibacterial activity; carboxymethyl-cellulose; composite; cytotoxicity; silver nanoparticles.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
HAADF-STEM images obtained from composites samples: (a) 0.7Ag1; (b) 0.7Ag2; (c) 0.7Ag3; and (d) 0.7Ag4. The particle size distribution of each sample is shown just below its HAADF-STEM image.
Figure 2
Figure 2
HAADF-STEM images obtained from composites samples: (a) 0.9Ag1; (b) 0.9Ag2; (c) 0.9Ag3; and (d) 0.9Ag4. The particle size distribution of each sample is shown just below its HAADF-STEM image.
Figure 3
Figure 3
HAADF-STEM images obtained from composites samples: (a) 1.2Ag1; (b) 1.2Ag2; (c) 1.2Ag3; and (d) 1.2Ag4. The particle size distribution of each sample is shown just below its HAADF-STEM image.
Figure 4
Figure 4
BF images and SAED patterns obtained from samples: (a) and (b) 0.7Ag1; (c) and (d) 0.9Ag1; (e) and (f) 1.2Ag1.
Figure 5
Figure 5
UV-vis spectra measured from (a) AgNO3; (b) 0.7Ag4; (c) 0.9Ag4; and (d) 1.2Ag4.
Figure 6
Figure 6
ATR-FTIR spectra obtained from (a) pure 0.7CMC and its composite samples; (b) pure 0.9CMC and its composite samples; (c) pure 1.2CMC and its composite samples.
Figure 7
Figure 7
Raman spectra recorded from: (a) 0.7Ag1; (b) 0.7Ag4; (c) 1.2Ag1; and (d) 1.2Ag2.
Figure 8
Figure 8
C1s and O1s spectra recorded by XPS from: (a) 0.7CMC; (b) 0.9CMC; and (c) 1.2CMC.
Figure 9
Figure 9
C1s and O1s spectra recorded by XPS from: (a) 0.7Ag1; (b) 0.9Ag1; and (c) 1.2Ag1.
Figure 10
Figure 10
C1s, O1s and Ag3d spectra recorded by XPS from samples: (a) 0.7Ag2; (b) 0.9Ag2; and (c) 1.2Ag2.
Figure 11
Figure 11
Antibacterial activity of CMC-AgNPs against E. faecalis growth. The data represent the percentage mean ± the percentage deviation.
Figure 12
Figure 12
Cytotoxicity obtained from CMC-AgNPs composites against ATCC®PCS-201-018™ cell line. The data represent the percentage mean ± the percentage deviation.
See this image and copyright information in PMC

References

    1. Stathopoulou M.-E.K., Banti C.N., Kourkoumelis N., Hatzidimitriou A.G., Kalampounias A.G., Hadjikakou S.K. Silver complex of salicylic acid and its hydrogel-cream in wound healing chemotherapy. J. Inorg. Biochem. 2018;181:41–55. doi: 10.1016/j.jinorgbio.2018.01.004. - DOI - PubMed
    1. Prucek R., Tuček J., Kilianová M., Panáček A., Kvítek L., Filip J., Kolář M., Tománková K., Zbořil R. The targeted antibacterial and antifungal properties of magnetic nanocomposite of iron oxide and silver nanoparticles. Biomaterials. 2011;32:4704–4713. doi: 10.1016/j.biomaterials.2011.03.039. - DOI - PubMed
    1. Carbone M., Donia D.T., Sabbatella G., Antiochia R. Silver nanoparticles in polymeric matrices for fresh food packaging. J. King Saud Univ. Sci. 2016;28:273–279. doi: 10.1016/j.jksus.2016.05.004. - DOI
    1. Farshchi E., Pirsa S., Roufegarinejad L., Alizadeh M., Rezazad M. Photocatalytic/biodegradable film based on carboxymethyl cellulose, modified by gelatin and TiO2-Ag nanoparticles. Carbohydr. Polym. 2019;216:189–196. doi: 10.1016/j.carbpol.2019.03.094. - DOI - PubMed
    1. Morones J.R., Elechiguerra J.L., Camacho A., Holt K., Kouri J.B., Ramírez J.T., Yacaman M.J. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16:2346–2353. doi: 10.1088/0957-4484/16/10/059. - DOI - PubMed

LinkOut - more resources