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. 2020 Jun;51(2):426-432.
doi: 10.1638/2019-0084.

CONSERVATION CHALLENGES: THE LIMITATIONS OF ANTEMORTEM TUBERCULOSIS TESTING IN CAPTIVE ASIATIC LIONS (PANTHERA LEO PERSICA)

Fieke Marije Molenaar  1 , Paul D Burr  2 , Benjamin M C Swift  3 , Catherine E D Rees  4 , Nicholas Masters
Affiliations

CONSERVATION CHALLENGES: THE LIMITATIONS OF ANTEMORTEM TUBERCULOSIS TESTING IN CAPTIVE ASIATIC LIONS (PANTHERA LEO PERSICA)

Fieke Marije Molenaar et al. J Zoo Wildl Med. 2020 Jun.

Abstract

Genetic diversity of captive wild animals can be enhanced by moving those individuals with valuable genes between collections and through introduction of a new pair from a range country. This requires movement of animals, which is inherent with disease risks, such as the introduction of pathogenic Mycobacterium sp. (MTBC) into a zoological collection. Decisions need to be made based on the outcome of perimovement disease screening using an array of tests, the majority of which are unvalidated in the species. A pair of endangered Asiatic lions (Panthera leo persica) imported from India to the United Kingdom were screened for MTBC using the comparative intradermal tuberculosis (TB) test, the feline interferon-γ blood test, and the experimental bacteriophage assay. Reactions on all three tests prompted screening of the three resident Asiatic lions using the same tests, all of which were negative for MTBC. Based on these test results, the decision had to be made to exclude the genetically valuable pair from the current collection. MTBC could not be identified using further tests, including culture and PCR on a bronchoalveolar lavage, on feces, or on postmortem tissues. This case series highlights the usefulness of a control group when interpreting unvalidated test results for detection of MTBC, the value of training big cats for conscious blood sampling, and the practical implications of placing the comparative intradermal TB test in the eyelids, when dealing with a species that requires a general anesthetic for most hands-on interventions.

Keywords: Asiatic lions; Panthera leo persica; TB testing; bacteriophage assay; comparative intradermal; interferon-γ.

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References

    1. Agren E, Gavier-Widen D. Confirming tuberculosis in zoo animals: the pathologist's perspective. In: Proc European Association of Zoo and Wildlife Veterinarians; 2008. p. 141.
    1. Bengis RG, Kriek NP, Keet DF, Raath JP, de Vos V, Huchzermeyer HF. An outbreak of bovine tuberculosis in a free-living African buffalo (Syncerus caffersparrman) population in the Kruger National Park: a preliminary report. Onderstepoort J Vet Res. 1996; 63(1):15–18.
    1. Bernitz N, Clarke C, Roos EO, Goosen WJ, Cooper D, van Helden PD, Parsons SDC, Miller MA. Detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer) using QuantiFERON((R))TB Gold (QFT) tubes and the Qiagen cattletype((R)) IFN-gamma ELISA. Vet Immunol Immunopathol. 2018;196:48–52.
    1. Breitenmoser U, Mallon DP, Ahmad Khan J, Driscoll C. International Union for the Conservation of Nature Red List of Threatened Species: Panthera leo persica [Internet]. 2008. Available from http://dx.doi.org/10.2305/IUCN.UK.2008.RLTS.T15952A5327221.en
    1. Bruns AC, Tanner M, Williams MC, Botha L, O'Brien A, Fosgate GT, van Helden PD, Clarke J, Michel AL. Diagnosis and implications of Mycobacterium bovis infection in banded mongooses (Mungos mungo) in the Kruger National Park, South Africa. J Wildl Dis. 2017;53(1):19–29.

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