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. 2019 Aug 1:2019:10.17912/micropub.biology.000141.
doi: 10.17912/micropub.biology.000141.

Generation of sas-6::ha by CRISPR/Cas9 editing

Mary Bergwell  1 , Amy Smith  1 , Holly Lakin  1 , Rebecca Slay  1 , Jyoti Iyer  1
Affiliations

Generation of sas-6::ha by CRISPR/Cas9 editing

Mary Bergwell et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1. Generation of IYR001 strain containing <i>sas-6(luv1[sas-6::ha])</i>, and its localization in the early <i>C. elegans</i> embryo
Figure 1. Generation of IYR001 strain containing sas-6(luv1[sas-6::ha]), and its localization in the early C. elegans embryo
A) Left panel: Cartoon depicting nucleotide sequences flanking the ha-tag sequence in the designed repair template to generate the sas-6::ha Red text- Silent mutations introduced in the repair template; Teal highlight- part of homology arms. Right panel: Cartoon depicting DNA fragment sizes expected upon PCR followed by NdeI digestion for wild-type and sas-6::ha edited worms. B) Agarose gel electrophoresis of NdeI digested sas-6 PCR products to screen for homozygous edited sas-6(luv1[sas-6::ha]) worms. L: 1Kb DNA ladder, Yellow stars: sas-6::ha homozygous worms. C) Immunostaining with an anti-HA antibody shows localization of SAS-6::HA to the centrosomes (insets) and in the cytoplasm in 1-cell C. elegans embryos during different stages of mitosis. Microtubules (red), SAS-6::HA (green) and DNA (blue).
See this image and copyright information in PMC

References

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