Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Aug 19:2019:10.17912/micropub.biology.000143.
doi: 10.17912/micropub.biology.000143.

Phase-separated protein dynamics are affected by fluorescent tag choice

Celja Uebel  1 , Carolyn Phillips  1
Affiliations

Phase-separated protein dynamics are affected by fluorescent tag choice

Celja Uebel et al. MicroPubl Biol. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Figure 1. PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. (A) Representative live images of endogenously tagged PGL-1::GFP (top row), PGL-1::mKate2 (middle row), and PGL-1::mTagBFP2 (bottom row) expression in the late pachytene and diplotene region of undissected C. elegans gonads. Scale bar, 10µm. (B) Live images of endogenously tagged PGL-1 in late pachytene zone of buffer dissected gonad. Scale bars, 5µm. (C) Live images of late pachytene region of gonads dissected in 5% 1,6-hexanediol, an aliphatic alcohol. Scale bars, 5µm. (D) Live images of undissected late pachytene region immediately after heat shock of 34°C for 3 hours. Scale bars, 5µm. (E) Live images of pachytene region 5 hours after microinjection of 200µg/mL of the transcriptional inhibitor, α-amanitin. Scale bars, 5µm.
See this image and copyright information in PMC

References

    1. Alberti S, Gladfelter A, Mittag T. Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates. Cell. 2019 Jan 24;176(3):419–434. doi: 10.1016/j.cell.2018.12.035. - DOI - PMC - PubMed
    1. Brangwynne CP, Eckmann CR, Courson DS, Rybarska A, Hoege C, Gharakhani J, Jülicher F, Hyman AA. Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science. 2009 May 21;324(5935):1729–1732. doi: 10.1126/science.1172046. - DOI - PubMed
    1. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B. Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Jun 01;200(4):1035–1049. doi: 10.1534/genetics.115.178335. - DOI - PMC - PubMed
    1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009 Apr 12;6(5):343–345. doi: 10.1038/nmeth.1318. - DOI - PubMed
    1. Kroschwald, S., Maharana, S., and Simon, A. Hexanediol: a chemical probe to investigate the material properties of membrane-less compartments. Matters 2017. 1–7.

LinkOut - more resources