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. 2019 Sep 3:2019:10.17912/micropub.biology.000159.
doi: 10.17912/micropub.biology.000159.

Identification of four Drosophila Toll-related proteins as ligands for the PTP69D receptor tyrosine phosphatase

Namrata Bali  1 , Hyung-Kook Lee  1 , Kai Zinn  1
Affiliations

Identification of four Drosophila Toll-related proteins as ligands for the PTP69D receptor tyrosine phosphatase

Namrata Bali et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1. Toll-2, Toll-6, Toll-7 and Toll-8 bind to PTP69D
Figure 1. Toll-2, Toll-6, Toll-7 and Toll-8 bind to PTP69D
(A) Binding patterns of Toll-2 AP, Toll-6 AP, Toll-7 AP and Toll-8 AP fusion proteins in wild type embryos. ai-aiv show double staining for FasII (green) and Toll-x AP (red). In ai’-aiv’ only the AP signal is shown, in white. All four fusion proteins show a similar binding pattern in the CNS with AP staining seen in longitudinal CNS axons, junctions between the longitudinal and the commissural tracts (yellow arrowheads), and in the posterior commissure (white arrows). Weak binding is also seen in the anterior commissure and in motor axons leaving the CNS. (B) Toll-2, Toll-6, Toll-7 and Toll-8 bind to overexpressed PTP69D. Wild type embryos (bi – biv) and embryos with overexpressed PTP69D (bi’ – biv’) were incubated with Toll-2 AP, Toll-6 AP, Toll-7 AP and Toll-8 AP fusion proteins. Here only the AP signal is shown, with black and white reversed relative to (a), so that AP staining is black. Embryos with ectopic PTP69D show increased binding of AP fusion proteins compared to wild type embryos. (C) PTP69D binds to overexpressed Toll-8. Wild type embryos (ci) and embryos with ectopic Toll-8 (cii) were incubated with PTP69D-AP fusion protein. Embryos with overexpressed Toll-8 show increased binding by PTP69D-AP (black) both in the CNS and in the periphery. Scale bars, 10µm.
See this image and copyright information in PMC

References

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