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. 2019 Apr 26:2019:10.17912/micropub.biology.000108.
doi: 10.17912/micropub.biology.000108.

me98 is a new allele of rad-54

Baptiste Roelens  1 , Karl Zawadzki  1 , Anne Villeneuve  1
Affiliations

me98 is a new allele of rad-54

Baptiste Roelens et al. MicroPubl Biol. .
No abstract available

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Figures

Figure 1.
Figure 1.
DAPI stained chromosomes in oocytes of the indicated genotype at diakinesis, the last stage of meiotic prophase; while six pairs of attached homologs are detected in wild-type animals, chromosome structural defects such as fragments (blue arrowhead) or partially decondensed chromosomes (purple arrowhead) are observed in the me98mutant. B. Detection of the crossover site marker GFP::COSA-1 in WT (left) and me98 mutant germ-cells (right). Scale bars on panels A and B represent 2µm. C. Detection of the recombinase RAD-51 in the region of the gonad corresponding to early to middle stages of meiotic prophase in WT (left) and me98 mutant (right) worms. RAD-51 foci are transiently detected in wild-type animals in early prophase as DNA breaks are formed and repair progresses. In contrast, in me98 mutants, RAD-51 foci accumulate and remain at high levels. Scale bar represents 10µm. D. Position and nature of the me98 deletion, located in the second exon of the rad-54 coding sequence, and its consequences on the encoded protein product. E. Screenshot of genome browser centered on the rad-54 locus. Positions of the previously described rad-54 allele, ok615, and the newly identified me98 mutation are represented respectively as a yellow and a red bar.
See this image and copyright information in PMC

References

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