Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jun 9:23:100772.
doi: 10.1016/j.bbrep.2020.100772. eCollection 2020 Sep.

In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)

Niloy Barua  1   2 , Md Arfin Ibn Aziz  1   2 , Abu Montakim Tareq  1 , Mohammed Aktar Sayeed  1 , Najmul Alam  1   2 , Nobi Ul Alam  1 , Mohammad Amran Uddin  1 , Chadni Lyzu  3 , Talha Bin Emran  2   4
Affiliations

In vivo and in vitro evaluation of pharmacological activities of Adenia trilobata (Roxb.)

Niloy Barua et al. Biochem Biophys Rep. .

Abstract

Adenia trilobata, locally known as akandaphal in Bangladesh, has some traditional uses. Leaves and stems extracted with pure methanol (MEATL, MEATS) and fractioned by n-hexane (NFATL, NFATS), which was subjected to qualitative phytochemical analysis. The qualitative phytochemical analysis of four extracts showed the presence of secondary metabolites such as alkaloid, carbohydrate, glycosides, flavonoids, phenols, flavonol, and saponins. All four extracts of A. trilobata, exhibited a strong antioxidant activity while a moderately (MEATS = 328 μg/mL) to weakly toxic (NFATL = 616.85 μg/mL) LC50 observed in brine shrimp lethality bioassay. In thrombolytic test, MEATL (18.54 ± 2.18%; P < 0.01) and MEATS (25.58 ± 4.76%; P < 0.0001) showed significant percentage of clot lysis in human blood. The in vivo analgesic activity carried by acetic acid test and formalin test, while the antidiarrheal activity assayed by two standard methods e.g., castor oil-induced diarrhea and castor oil-induced gastrointestinal motility. Both, in vivo model, showed an extremely significant (P < 0.0001) dose-dependent manner percentage of inhibition in comparison to the control group. Present results suggested, A. trilobata could be a potential source for antioxidative, cytotoxic, thrombolytic, analgesic, antidiarrheal agents which require further study to identify the mechanism of A. trilobata.

Keywords: A. trilobata; A. trilobata, Adenia trilobata; ANOVA, Analysis of variance; Analgesic activity; Antidiarrheal; Antioxidant; Cytotoxic; DPPH, 1,1-diphenyl, 2-picryl hydrazyl; FCR, Folin-Ciocalteu reagent; IC50, 50% inhibitory concentration; IP, intraperitoneal; LC50, 50% lethal concentration; OS, oxidative stress; SEM, standard error mean; Thrombolytic; UV, ultra-violet; b.w., body weight.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Percentage of radical scavenging activities the DPPH assay of A. trilobata fractions and standard drug at different concentrations. Values are represented in Mean ± SEM (n = 3). b P < 0.01, c P < 0.001 and d P < 0.0001 are statistically significant in comparison to ascorbic acid followed by unpaired t-test of one-way ANOVA (GraphPad Prism 7). MEATL: Methanolic extract of A. trilobata leaves, NFATL: n-hexane fraction of A. trilobata leaves, MEATS: Methanolic extract of A. trilobata stem and NFATS: n-hexane fraction of A. trilobata stem.
Fig. 2
Fig. 2
Reducing power of A. trilobata fractions and standard drug at different concentrations. MEATL: Methanolic extract of A. trilobata leaves, NFATL: n-hexane fraction of A. trilobata leaves, MEATS: Methanolic extract of A. trilobata stem and NFATS: n-hexane fraction of A. trilobata stem.
Fig. 3
Fig. 3
Percentage of mortality of brine shrimp of A. trilobata fractions and standard drug at different concentrations. MEATL: Methanolic extract of A. trilobata leaves, NFATL: n-hexane fraction of A. trilobata leaves, MEATS: Methanolic extract of A. trilobata stem and NFATs: n-hexane fraction of A. trilobata stem.
Fig. 4
Fig. 4
Percentage of clot lysis of human blood by A. trilobata fractions and standard drug. Values are represented in Mean ± SEM (n = 6). b P < 0.01 and d P < 0.0001 are statistically significant in comparison to negative control (water) followed by unpaired t-test of one-way ANOVA (GraphPad Prism 7). SK: streptokinase, MEATL: Methanolic extract of A. trilobata leaves, NFATL: n-hexane fraction of A. trilobata leaves, MEATS: Methanolic extract of A. trilobata stem and NFATS: n-hexane fraction of A. trilobata stem.
See this image and copyright information in PMC

References

    1. Mendonça-Junior F.J., Scotti M.T., Nayarisseri A., Zondegoumba E.N., Scotti L. Natural bioactive products with antioxidant properties useful in neurodegenerative diseases. Oxi. Med. Cell. Longev. 2019:1–2. 7151780. - PMC - PubMed
    1. Festa F., Aglitti T., Duranti G., Ricordy R., Perticone P., Cozzi R. Strong antioxidant activity of ellagic acid in mammalian cells in vitro revealed by the comet assay. Anticancer Res. 2001;21(6A):3903–3908. - PubMed
    1. Nicolini F.A., Nichols W.W., Mehta J.L., Saldeen T.G., Schofield R., Ross M., Player D.W., Pohl G.B., Mattsson C. Sustained reflow in dogs with coronary thrombosis with K2P, a novel mutant of tissue-plasminogen activator. J. Am. Coll. Cardiol. 1992;20(1):228–235. - PubMed
    1. Milind P., Monu Y. Laboratory models for screening analgesics. Int. Res. J. Pharm. 2013;4:15–19.
    1. Kumar M., Shek A., Akbar Z. A review on analgesic: from natural sources. Int. J. Pharm. Biol. Arch. 2010;1:95–100.

LinkOut - more resources