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. 2020 Dec;9(1):1393-1396.
doi: 10.1080/22221751.2020.1775500.

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Fabiola Mancini  1 , Fabrizio Barbanti  1 , Maria Scaturro  1 , Giulia Errico  1   2 , Angelo Iacobino  1 , Antonino Bella  1 , Flavia Riccardo  1 , Giulia Marsili  1 , Paola Stefanelli  1 , Patrizio Pezzotti  1 , Giovanni Rezza  1 , Alessandra Ciervo; on behalf ISS COVID-19 study group  1
Affiliations

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Fabiola Mancini et al. Emerg Microbes Infect. 2020 Dec.

Abstract

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

Keywords: COVID 19; SARS-CoV-2; heat treatment; molecular methods; nasopharyngeal samples; rt-Real time PCR.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

References

    1. WHO . Disease outbreak news. Emergencies preparedness, response. Pneumonia of unknown origin – China. Word Health Organization. 2020. Available from: https://www.who.int/csr/don/05-january-2020-pneumonia-of-unkown-cause-ch....
    1. Wang C, Horby PW, Hayden FG, et al. . A novel coronavirus outbreak of global health concern. The Lancet. 2020;395(10223):470–473. doi:10.1016/S0140-6736(20)30185-9. - DOI - PMC - PubMed
    1. Gorbalenya AE, Baker SC, Baric RS, et al. . Severe acute respiratory syndrome-related coronavirus: the species and its viruses—a statement of the coronavirus study group. bioRxiv. 2020.02.07.93786. doi: 10.1101/2020.02.07.937862 - DOI
    1. Coronaviridae Study Group of the International Committee on Taxonomy of Viruses . The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Nat Microbiol. 2020;5:536–544. doi:10.1038/s41564-020-0695-z. - DOI - PMC - PubMed
    1. WHO. Molecular assays to diagnose COVID-19 . Word Health Organization. 2020. Available from: https://www.who.int/docs/default-source/coronaviruse/whoinhouseassays.pd....