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. 2020 Jun 17;13(1):295.
doi: 10.1186/s13104-020-05138-7.

Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

Eiman Siddig Ahmed  1 , Lubna S Elnour  2 , Rowa Hassan  1 , Emmanuel E Siddig  1   2   3   4 , Mintu Elsa Chacko  5 , Eman T Ali  2   6 , Mona A Mohamed  7 , Abdalla Munir  8 , Mohamed S Muneer  9   10   11 , Nouh S Mohamed  12   13   14 , Ali M M Edris  2   15
Affiliations

Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

Eiman Siddig Ahmed et al. BMC Res Notes. .

Abstract

Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. The study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system.

Results: A total of 153 males' prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

Keywords: Cyclin D1; Immunohistochemical expression; Prognostic markers; Prostate cancer.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The correlation of Cyclin D1 immunohistochemistry expression in BPH and PC. a shows the level of expression of cyclin D1 in benign prostatic hyperplasia (BPH), b shows the high expression level of Cyclin D1 in prostate cancer (PC)
Fig. 2
Fig. 2
Photomicroscopy from representative Cyclin D1 immunohistochemistry expression; a and b indicates low-grade expression; 10% to 25% of the cells were expressing the Cyclin D1. c and d indicates high-grade expression, in C: (> 25–75%) of the cells showed expression of the marker. in d more than 75% of the cells expressed the marker
See this image and copyright information in PMC

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