Scalable and robust SARS-CoV-2 testing in an academic center

Jim Aitken¹, Karen Ambrose¹, Sam Barrell¹, Rupert Beale^{1

2}, Ganka Bineva-Todd¹, Dhruva Biswas², Richard Byrne¹, Simon Caidan¹, Peter Cherepanov¹, Laura Churchward³, Graham Clark¹, Margaret Crawford¹, Laura Cubitt¹, Vicky Dearing¹, Christopher Earl¹, Amelia Edwards¹, Chris Ekin⁴, Efthymios Fidanis¹, Alessandra Gaiba¹, Steve Gamblin⁵, Sonia Gandhi^{6

7

8}, Jacki Goldman¹, Robert Goldstone¹, Paul R Grant^#⁴, Maria Greco¹, Judith Heaney³, Steve Hindmarsh¹, Catherine F Houlihan³, Michael Howell¹, Michael Hubank^{9

10}, Deborah Hughes⁹, Rachael Instrell¹, Deborah Jackson¹, Mariam Jamal-Hanjani^{2

3}, Ming Jiang¹, Mark Johnson¹, Leigh Jones¹, Nnennaya Kanu^#², George Kassiotis¹, Stuart Kirk⁴, Svend Kjaer¹, Andrew Levett⁴, Lisa Levett⁴, Marcel Levi³, Wei-Ting Lu¹, James I MacRae¹, John Matthews⁴, Laura E McCoy², Catherine Moore¹¹, David Moore^{2

3}, Eleni Nastouli^{12

13}, Jerome Nicod¹, Luke Nightingale¹, Jessica Olsen¹, Nicola O'Reilly¹, Amar Pabari⁴, Venizelos Papayannopoulos¹, Namita Patel¹, Nigel Peat¹, Marc Pollitt¹, Peter Ratcliffe¹, Caetano Reis e Sousa¹, Annachiara Rosa¹, Rachel Rosenthal¹, Chloe Roustan¹, Andrew Rowan¹, Gee Yen Shin^{3

4}, Daniel M Snell¹, Ok-Ryul Song¹, Moira J Spyer², Amy Strange¹, Charles Swanton^{14

15

16}, James M A Turner¹, Melanie Turner⁴, Andreas Wack¹, Philip A Walker¹, Sophia Ward^{1

2}, Wai Keong Wong³, Joshua Wright¹, Mary Wu¹; Crick COVID-19 Consortium

Collaborators, Affiliations

Collaborators

Crick COVID-19 Consortium:
Jim Aitken, Zoe Allen, Rachel Ambler, Karen Ambrose, Emma Ashton, Alida Avola, Samutheswari Balakrishnan, Caitlin Barns-Jenkins, Genevieve Barr, Sam Barrell, Souradeep Basu, Rupert Beale, Clare Beesley, Nisha Bhardwaj, Shahnaz Bibi, Ganka Bineva-Todd, Dhruva Biswas, Michael J Blackman, Dominique Bonnet, Faye Bowker, Malgorzata Broncel, Claire Brooks, Michael D Buck, Andrew Buckton, Timothy Budd, Alana Burrell, Louise Busby, Claudio Bussi, Simon Butterworth, Fiona Byrne, Richard Byrne, Simon Caidan, Joanna Campbell, Johnathan Canton, Ana Cardoso, Nick Carter, Luiz Carvalho, Raffaella Carzaniga, Natalie Chandler, Qu Chen, Peter Cherepanov, Laura Churchward, Graham Clark, Bobbi Clayton, Clementina Cobolli Gigli, Zena Collins, Sally Cottrell, Margaret Crawford, Laura Cubitt, Tom Cullup, Heledd Davies, Patrick Davis, Dara Davison, Vicky Dearing, Solene Debaisieux, Monica Diaz-Romero, Alison Dibbs, Jessica Diring, Paul C Driscoll, Annalisa D'Avola, Christopher Earl, Amelia Edwards, Chris Ekin, Dimitrios Evangelopoulos, Rupert Faraway, Antony Fearns, Aaron Ferron, Efthymios Fidanis, Dan Fitz, James Fleming, Bruno Frederico, Alessandra Gaiba, Anthony Gait, Steve Gamblin, Sonia Gandhi, Liam Gaul, Helen M Golding, Jacki Goldman, Robert Goldstone, Belen Gomez Dominguez, Hui Gong, Paul R Grant, Maria Greco, Mariana Grobler, Anabel Guedan, Maximiliano G Gutierrez, Fiona Hackett, Ross Hall, Steinar Halldorsson, Suzanne Harris, Sugera Hashim, Lyn Healy, Judith Heaney, Susanne Herbst, Graeme Hewitt, Theresa Higgins, Steve Hindmarsh, Rajnika Hirani, Joshua Hope, Elizabeth Horton, Beth Hoskins, Catherine F Houlihan, Michael Howell, Louise Howitt, Jacqueline Hoyle, Mint R Htun, Michael Hubank, Hector Huerga Encabo, Deborah Hughes, Jane Hughes, Almaz Huseynova, Ming-Shih Hwang, Rachael Instrell, Deborah Jackson, Mariam Jamal-Hanjani, Lucy Jenkins, Ming Jiang, Mark Johnson, Leigh Jones, Nnennaya Kanu, George Kassiotis, Louise Kiely, Anastacio King Spert Teixeira, Stuart Kirk, Svend Kjaer, Ellen Knuepfer, Nikita Komarov, Paul Kotzampaltiris, Konstantinos Kousis, Tammy Krylova, Ania Kucharska, Robyn Labrum, Catherine Lambe, Michelle Lappin, Stacey-Ann Lee, Andrew Levett, Lisa Levett, Marcel Levi, Hon Wing Liu, Sam Loughlin, Wei-Ting Lu, James I MacRae, Akshay Madoo, Julie A Marczak, Mimmi Martensson, Thomas Martinez, Bishara Marzook, John Matthews, Joachim M Matz, Samuel McCall, Laura E McCoy, Fiona McKay, Edel C McNamara, Carlos M Minutti, Gita Mistry, Miriam Molina-Arcas, Beatriz Montaner, Kylie Montgomery, Catherine Moore, David Moore, Anastasia Moraiti, Lucia Moreira-Teixeira, Joyita Mukherjee, Cristina Naceur-Lombardelli, Eleni Nastouli, Aileen Nelson, Jerome Nicod, Luke Nightingale, Stephanie Nofal, Paul Nurse, Savita Nutan, Caroline Oedekoven, Anne O'Garra, Jean D O'Leary, Jessica Olsen, Olga O'Neill, Nicola O'Reilly, Paula Ordonez Suarez, Neil Osborne, Amar Pabari, Aleksandra Pajak, Venizelos Papayannopoulos, Namita Patel, Yogen Patel, Oana Paun, Nigel Peat, Laura Peces-Barba Castano, Ana Perez Caballero, Jimena Perez-Lloret, Magali S Perrault, Abigail Perrin, Roy Poh, Enzo Z Poirier, James M Polke, Marc Pollitt, Lucia Prieto-Godino, Alize Proust, Clinda Puvirajasinghe, Christophe Queval, Vijaya Ramachandran, Abhinay Ramaprasad, Peter Ratcliffe, Laura Reed, Caetano Reis e Sousa, Kayleigh Richardson, Sophie Ridewood, Fiona Roberts, Rowenna Roberts, Angela Rodgers, Pablo Romero Clavijo, Annachiara Rosa, Rachel Rosenthal, Alice Rossi, Chloe Roustan, Andrew Rowan, Erik Sahai, Aaron Sait, Katarzyna Sala, Theo Sanderson, Pierre Santucci, Fatima Sardar, Adam Sateriale, Jill A Saunders, Chelsea Sawyer, Anja Schlott, Edina Schweighoffer, Sandra Segura-Bayona, Rajvee Shah Punatar, Joe Shaw, Gee Yen Shin, Mariana Silva Dos Santos, Margaux Silvestre, Matthew Singer, Daniel M Snell, Ok-Ryul Song, Moira J Spyer, Louisa Steel, Amy Strange, Adrienne E Sullivan, Charles Swanton, Michele S Y Tan, Zoe H Tautz-Davis, Effie Taylor, Gunes Taylor, Harriet B Taylor, Alison Taylor-Beadling, Fernanda Teixeira Subtil, Berta Terré Torras, Patrick Toolan-Kerr, Francesca Torelli, Tea Toteva, Moritz Treeck, Hadija Trojer, Ming-Han C Tsai, James M A Turner, Melanie Turner, Jernej Ule, Rachel Ulferts, Sharon P Vanloo, Selvaraju Veeriah, Subramanian Venkatesan, Karen Vousden, Andreas Wack, Claire Walder, Philip A Walker, Yiran Wang, Sophia Ward, Catharina Wenman, Luke Williams, Matthew J Williams, Wai Keong Wong, Joshua Wright, Mary Wu, Lauren Wynne, Zheng Xiang, Melvyn Yap, Julian A Zagalak, Davide Zecchin, Rachel Zillwood

Affiliations

¹ The Francis Crick Institute, London, UK.
² University College London, London, UK.
³ University College London Hospitals, NHS Foundation Trust, London, UK.
⁴ Health Services Laboratories, London, UK.
⁵ The Francis Crick Institute, London, UK. steve.gamblin@crick.ac.uk.
⁶ The Francis Crick Institute, London, UK. sonia.gandhi@crick.ac.uk.
⁷ University College London, London, UK. sonia.gandhi@crick.ac.uk.
⁸ University College London Hospitals, NHS Foundation Trust, London, UK. sonia.gandhi@crick.ac.uk.
⁹ The Institute of Cancer Research, London, UK.
¹⁰ The Royal Marsden Hospital, Surrey, UK.
¹¹ Public Health Wales, Heath Park, Cardiff, UK.
¹² University College London Hospitals, NHS Foundation Trust, London, UK. eleni.nastouli@nhs.net.
¹³ University College London GOS Institute of Child Health, London, UK. eleni.nastouli@nhs.net.
¹⁴ The Francis Crick Institute, London, UK. Charles.Swanton@crick.ac.uk.
¹⁵ University College London, London, UK. Charles.Swanton@crick.ac.uk.
¹⁶ University College London Hospitals, NHS Foundation Trust, London, UK. Charles.Swanton@crick.ac.uk.

^# Contributed equally.

PMID: 32555528
DOI: 10.1038/s41587-020-0588-y

Scalable and robust SARS-CoV-2 testing in an academic center

Jim Aitken et al. Nat Biotechnol. 2020 Aug.

. 2020 Aug;38(8):927-931.

doi: 10.1038/s41587-020-0588-y.

Authors

Collaborators

Crick COVID-19 Consortium:
Jim Aitken, Zoe Allen, Rachel Ambler, Karen Ambrose, Emma Ashton, Alida Avola, Samutheswari Balakrishnan, Caitlin Barns-Jenkins, Genevieve Barr, Sam Barrell, Souradeep Basu, Rupert Beale, Clare Beesley, Nisha Bhardwaj, Shahnaz Bibi, Ganka Bineva-Todd, Dhruva Biswas, Michael J Blackman, Dominique Bonnet, Faye Bowker, Malgorzata Broncel, Claire Brooks, Michael D Buck, Andrew Buckton, Timothy Budd, Alana Burrell, Louise Busby, Claudio Bussi, Simon Butterworth, Fiona Byrne, Richard Byrne, Simon Caidan, Joanna Campbell, Johnathan Canton, Ana Cardoso, Nick Carter, Luiz Carvalho, Raffaella Carzaniga, Natalie Chandler, Qu Chen, Peter Cherepanov, Laura Churchward, Graham Clark, Bobbi Clayton, Clementina Cobolli Gigli, Zena Collins, Sally Cottrell, Margaret Crawford, Laura Cubitt, Tom Cullup, Heledd Davies, Patrick Davis, Dara Davison, Vicky Dearing, Solene Debaisieux, Monica Diaz-Romero, Alison Dibbs, Jessica Diring, Paul C Driscoll, Annalisa D'Avola, Christopher Earl, Amelia Edwards, Chris Ekin, Dimitrios Evangelopoulos, Rupert Faraway, Antony Fearns, Aaron Ferron, Efthymios Fidanis, Dan Fitz, James Fleming, Bruno Frederico, Alessandra Gaiba, Anthony Gait, Steve Gamblin, Sonia Gandhi, Liam Gaul, Helen M Golding, Jacki Goldman, Robert Goldstone, Belen Gomez Dominguez, Hui Gong, Paul R Grant, Maria Greco, Mariana Grobler, Anabel Guedan, Maximiliano G Gutierrez, Fiona Hackett, Ross Hall, Steinar Halldorsson, Suzanne Harris, Sugera Hashim, Lyn Healy, Judith Heaney, Susanne Herbst, Graeme Hewitt, Theresa Higgins, Steve Hindmarsh, Rajnika Hirani, Joshua Hope, Elizabeth Horton, Beth Hoskins, Catherine F Houlihan, Michael Howell, Louise Howitt, Jacqueline Hoyle, Mint R Htun, Michael Hubank, Hector Huerga Encabo, Deborah Hughes, Jane Hughes, Almaz Huseynova, Ming-Shih Hwang, Rachael Instrell, Deborah Jackson, Mariam Jamal-Hanjani, Lucy Jenkins, Ming Jiang, Mark Johnson, Leigh Jones, Nnennaya Kanu, George Kassiotis, Louise Kiely, Anastacio King Spert Teixeira, Stuart Kirk, Svend Kjaer, Ellen Knuepfer, Nikita Komarov, Paul Kotzampaltiris, Konstantinos Kousis, Tammy Krylova, Ania Kucharska, Robyn Labrum, Catherine Lambe, Michelle Lappin, Stacey-Ann Lee, Andrew Levett, Lisa Levett, Marcel Levi, Hon Wing Liu, Sam Loughlin, Wei-Ting Lu, James I MacRae, Akshay Madoo, Julie A Marczak, Mimmi Martensson, Thomas Martinez, Bishara Marzook, John Matthews, Joachim M Matz, Samuel McCall, Laura E McCoy, Fiona McKay, Edel C McNamara, Carlos M Minutti, Gita Mistry, Miriam Molina-Arcas, Beatriz Montaner, Kylie Montgomery, Catherine Moore, David Moore, Anastasia Moraiti, Lucia Moreira-Teixeira, Joyita Mukherjee, Cristina Naceur-Lombardelli, Eleni Nastouli, Aileen Nelson, Jerome Nicod, Luke Nightingale, Stephanie Nofal, Paul Nurse, Savita Nutan, Caroline Oedekoven, Anne O'Garra, Jean D O'Leary, Jessica Olsen, Olga O'Neill, Nicola O'Reilly, Paula Ordonez Suarez, Neil Osborne, Amar Pabari, Aleksandra Pajak, Venizelos Papayannopoulos, Namita Patel, Yogen Patel, Oana Paun, Nigel Peat, Laura Peces-Barba Castano, Ana Perez Caballero, Jimena Perez-Lloret, Magali S Perrault, Abigail Perrin, Roy Poh, Enzo Z Poirier, James M Polke, Marc Pollitt, Lucia Prieto-Godino, Alize Proust, Clinda Puvirajasinghe, Christophe Queval, Vijaya Ramachandran, Abhinay Ramaprasad, Peter Ratcliffe, Laura Reed, Caetano Reis e Sousa, Kayleigh Richardson, Sophie Ridewood, Fiona Roberts, Rowenna Roberts, Angela Rodgers, Pablo Romero Clavijo, Annachiara Rosa, Rachel Rosenthal, Alice Rossi, Chloe Roustan, Andrew Rowan, Erik Sahai, Aaron Sait, Katarzyna Sala, Theo Sanderson, Pierre Santucci, Fatima Sardar, Adam Sateriale, Jill A Saunders, Chelsea Sawyer, Anja Schlott, Edina Schweighoffer, Sandra Segura-Bayona, Rajvee Shah Punatar, Joe Shaw, Gee Yen Shin, Mariana Silva Dos Santos, Margaux Silvestre, Matthew Singer, Daniel M Snell, Ok-Ryul Song, Moira J Spyer, Louisa Steel, Amy Strange, Adrienne E Sullivan, Charles Swanton, Michele S Y Tan, Zoe H Tautz-Davis, Effie Taylor, Gunes Taylor, Harriet B Taylor, Alison Taylor-Beadling, Fernanda Teixeira Subtil, Berta Terré Torras, Patrick Toolan-Kerr, Francesca Torelli, Tea Toteva, Moritz Treeck, Hadija Trojer, Ming-Han C Tsai, James M A Turner, Melanie Turner, Jernej Ule, Rachel Ulferts, Sharon P Vanloo, Selvaraju Veeriah, Subramanian Venkatesan, Karen Vousden, Andreas Wack, Claire Walder, Philip A Walker, Yiran Wang, Sophia Ward, Catharina Wenman, Luke Williams, Matthew J Williams, Wai Keong Wong, Joshua Wright, Mary Wu, Lauren Wynne, Zheng Xiang, Melvyn Yap, Julian A Zagalak, Davide Zecchin, Rachel Zillwood

Affiliations

¹ The Francis Crick Institute, London, UK.
² University College London, London, UK.
³ University College London Hospitals, NHS Foundation Trust, London, UK.
⁴ Health Services Laboratories, London, UK.
⁵ The Francis Crick Institute, London, UK. steve.gamblin@crick.ac.uk.
⁶ The Francis Crick Institute, London, UK. sonia.gandhi@crick.ac.uk.
⁷ University College London, London, UK. sonia.gandhi@crick.ac.uk.
⁸ University College London Hospitals, NHS Foundation Trust, London, UK. sonia.gandhi@crick.ac.uk.
⁹ The Institute of Cancer Research, London, UK.
¹⁰ The Royal Marsden Hospital, Surrey, UK.
¹¹ Public Health Wales, Heath Park, Cardiff, UK.
¹² University College London Hospitals, NHS Foundation Trust, London, UK. eleni.nastouli@nhs.net.
¹³ University College London GOS Institute of Child Health, London, UK. eleni.nastouli@nhs.net.
¹⁴ The Francis Crick Institute, London, UK. Charles.Swanton@crick.ac.uk.
¹⁵ University College London, London, UK. Charles.Swanton@crick.ac.uk.
¹⁶ University College London Hospitals, NHS Foundation Trust, London, UK. Charles.Swanton@crick.ac.uk.

^# Contributed equally.

PMID: 32555528
DOI: 10.1038/s41587-020-0588-y

Erratum in

Author Correction: Scalable and robust SARS-CoV-2 testing in an academic center.
Aitken J, Ambrose K, Barrell S, Beale R, Bineva-Todd G, Biswas D, Byrne R, Caidan S, Cherepanov P, Churchward L, Clark G, Crawford M, Cubitt L, Dearing V, Earl C, Edwards A, Ekin C, Fidanis E, Gaiba A, Gamblin S, Gandhi S, Goldman J, Goldstone R, Grant PR, Greco M, Heaney J, Hindmarsh S, Houlihan CF, Howell M, Hubank M, Hughes D, Instrell R, Jackson D, Jamal-Hanjani M, Jiang M, Johnson M, Jones L, Kanu N, Kassiotis G, Kirk S, Kjaer S, Levett A, Levett L, Levi M, Lu WT, MacRae JI, Matthews J, McCoy LE, Moore C, Moore D, Nastouli E, Nicod J, Nightingale L, Olsen J, O'Reilly N, Pabari A, Papayannopoulos V, Patel N, Peat N, Pollitt M, Ratcliffe P, Reis e Sousa C, Rosa A, Rosenthal R, Roustan C, Rowan A, Shin GY, Snell DM, Song OR, Spyer MJ, Strange A, Swanton C, Turner JMA, Turner M, Wack A, Walker PA, Ward S, Wong WK, Wright J, Wu M; Crick COVID-19 Consortium. Aitken J, et al. Nat Biotechnol. 2020 Aug;38(8):1000. doi: 10.1038/s41587-020-0623-z. Nat Biotechnol. 2020. PMID: 32681136 Free PMC article.

No abstract available

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References

1. Jiang, S., Du, L. & Shi, Z. Emerg. Microbes Infect. 9, 275–277 (2020). - DOI - PubMed - PMC
1. Zhou, P. et al. Nature 579, 270–273 (2020). - PubMed - PMC
1. Zhu, N. et al. N. Engl. J. Med. 382, 727–733 (2020). - DOI - PubMed - PMC
1. Crick COVID-19 Consortium. The Crick COVID-19 RT-PCR testing pipeline. protocols.io https://doi.org/10.17504/protocols.io.bfe2jjge (2020).
1. Boom, R. et al. J. Clin. Microbiol. 28, 495–503 (1990). - DOI - PubMed - PMC

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Scalable and robust SARS-CoV-2 testing in an academic center

Collaborators

Affiliations

Scalable and robust SARS-CoV-2 testing in an academic center

Authors

Collaborators

Affiliations

Erratum in

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Medical

Miscellaneous