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. 2020 Jul 25;56(58):8091-8094.
doi: 10.1039/d0cc01673j. Epub 2020 Jun 17.

An NMR based phosphodiesterase assay

Madoka Akimoto  1 , Tianning Yu, Kody Moleschi, Katherine Van, Ganesh S Anand, Giuseppe Melacini
Affiliations

An NMR based phosphodiesterase assay

Madoka Akimoto et al. Chem Commun (Camb). .

Abstract

We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.

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