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. 2020 Oct;52(9):e13713.
doi: 10.1111/and.13713. Epub 2020 Jun 17.

Heat-induced changes in the expression and localisation of PGC-1α in the mice testis

Milirani Das  1 , Chuckles Ch Marak  1 , Malsawmhriatzuala Jeremy  1 , Guruswami Gurusubramanian  1 , Vikas Kumar Roy  1
Affiliations

Heat-induced changes in the expression and localisation of PGC-1α in the mice testis

Milirani Das et al. Andrologia. 2020 Oct.

Abstract

The functions of mammalian testis are temperature-sensitive. There are various testicular factors, which express in response to heat as a mechanism of defence. PGC-1α and HSP70 have poetical role in the protection from oxidative stress in various tissues, including testis. The expression of PGC-1α and HSP70 has been shown in the testis, and it has also been documented that heat modulates the expression of PGC-1α and HSP70. However, heat-dependent changes in the localisation and expression of PGC-1α have not been investigated so far. Thus, we studied the expression and localisation pattern of PGC-1α in the testis of heat-treated mice along with marker of proliferation (PCNA, GCNA), serum testosterone levels, MDA levels and HSP70. The results showed a significant increase in PGC-1α and HSP70 and MDA levels in the testis of heat-treated mice along with a decrease in PCNA, GCNA and serum testosterone levels. The immunolocalisation study showed intense immunostaining of PGC-1α in the Leydig cell and germ cells of the heat-treated testis, with pronounced damaged in the histoarchitecture. The results showed that increase expression of PGC-1α in germ cells and Leydig cells of testis could be a counter mechanism to cope up with oxidative stress in coordination with HSP70.

Keywords: HSP70; PGC-1α; heat stress; testis.

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