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. 2021 Jan;19(1):11-13.
doi: 10.1111/pbi.13433. Epub 2020 Jul 3.

Fine-tuning the amylose content of rice by precise base editing of the Wx gene

Yang Xu  1   2 , Qiupeng Lin  3   4 , Xiufeng Li  5 , Fangquan Wang  1   2 , Zhihui Chen  1   2 , Jun Wang  1   2 , Wenqi Li  1   2 , Fangjun Fan  1   2 , Yajun Tao  1   2 , Yanjie Jiang  1   2 , Xiaodong Wei  1 , Rui Zhang  3 , Qian-Hao Zhu  6 , Qingyun Bu  5 , Jie Yang  1   2 , Caixia Gao  3   4
Affiliations

Fine-tuning the amylose content of rice by precise base editing of the Wx gene

Yang Xu et al. Plant Biotechnol J. 2021 Jan.
No abstract available

Keywords: Wx; amylose content; appearance quality; base editing; eating and cooking quality; rice.

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Conflict of interest statement

The authors have submitted a patent application based on the results reported in this paper.

Figures

Figure 1
Figure 1
Fine‐tuning amylose content by precise base editing of Wx in rice. (a) Diagram of the target Wxb gene. (b) Mutations in the edited T0 and T1 lines. The putative protospacer‐adjacent motifs (PAMs) are shown in green. The number of altered bases in each line (coloured in red) is indicated by the letter S followed by a number. (c) A structural model of Wxb constructed using the PROTEIN DATA BANK server; mutated residues contributing to the changes of AC are shown as spheres and are coloured (P124 in apricot, R125 in blue, R158 in red violet, G159 in white, V160 in green, D161 in red, T178 in orange and Y191 in purple). (d) Analysis of potential off‐target sites in the seven T1 edited lines. Red lower‐case bases are mismatches to TS1‐TS3; +, mutations detected; −, mutations not detected. (e) The AACs determined by iodine colorimetry of NIP, NG9108 and the edited lines. (f) GBSSI activities of the seeds of NIP and the edited lines. (g) SDS‐PAGE analysis of starch granule‐bound GBSSI (top) and total seed proteins (bottom) from mature seeds. M is a protein marker. (h) Comparisons of the appearance of different forms of milled rice. Scale bars, 1.75 cm. (i) The AACs of the two japonica varieties JG818, SJ18 and their corresponding Wx‐edited lines. The different letters in (e), (f) and (i) indicate significant differences at P < 0.05 by Student's t‐test. Data are means ± SD (n = 3).
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