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. 2020 Nov;22(11):e3243.
doi: 10.1002/jgm.3243. Epub 2020 Jul 10.

COMMD8 changes expression during initial phase of wasp venom immunotherapy

Karol Kempiński  1 , Jan Romantowski  1 , Agnieszka Maciejewska  2 , Ryszard Pawłowski  2 , Marta Chełmińska  1 , Ewa Jassem  1 , Marek Niedoszytko  1
Affiliations

COMMD8 changes expression during initial phase of wasp venom immunotherapy

Karol Kempiński et al. J Gene Med. 2020 Nov.

Abstract

Background: Hymenoptera venom allergy (HVA) is of great concern because of the possibility of anaphylaxis, which may be fatal. Venom immunotherapy (VIT) is the only disease-modifying treatment in HVA and, although efficient, its mechanism remains partially unknown. Gene expression analysis may be helpful for establishing a proper model of tolerance induction during the build-up phase of VIT. The present study aimed to analyze how the start of VIT changes the expression of 15 selected genes.

Methods: Forty-five patients starting VIT with a wasp venom allergy were enrolled. The diagnosis was established based on anaphylaxis history (third or fourth grade on the Mueller scale) and positive soluble immunoglobulin E and/or skin tests. Two blood collections were performed in the patient group: before and after 3 months of VIT. One sample was taken in the control group. Gene expression analysis was performed using a reverse transcriptase-polymerase chain reaction with microfluidic cards and normalized to the 18S housekeeping gene.

Results: Commd8 was the only gene that changed expression significantly after the start of VIT (p = 0.012). Its expression decreased towards the levels observed in the healthy controls. Twelve out of 15 genes (commd8, cldn1, cngb3, fads1, hes6, hla-drb5, htr3b, prlr, slc16a4, snx33, socs3 and twist2) revealed a significantly different expression compared to the healthy controls.

Conclusions: The present study shows that commd8 changes significantly its expression during initial phase of VIT. This gene might be a candidate for VIT biomarker in future studies.

Keywords: RNA; allergy; gene expression; immune response.

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References

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