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Comment
. 2020 Sep;30(9):715-716.
doi: 10.1038/s41422-020-0360-3.

Seeing is believing: a novel tool for quantitating mitophagy

Liming Wang  1 , Han-Ming Shen  2
Affiliations
Comment

Seeing is believing: a novel tool for quantitating mitophagy

Liming Wang et al. Cell Res. 2020 Sep.
No abstract available

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Figures

Fig. 1
Fig. 1. Illustration of how to use mito-SRAI to analyze mitophagic flux.
Mito-SRAI is a tandem construct containing a MMTS and two fluorescent protein tags, YPet and TOLLES. When mitochondria are healthy, mito-SRAI emits both yellow and cyan fluorescence in mitochondria matrix (pH ~8). When mitochondria are damaged, mitochondria are engulfed by autophagosomes and delivered to lysosomes to form mitolysosomes. Within the acidic environment of mitolysosomes (pH ~4.5), the YPet (yellow fluorescence) is irreversibly quenched and/or digested, whereas TOLLES remains completely intact and emits full cyan fluorescence. Thus, the ratio (TOLLES vs. YPet) can be used as an index of mitophagic activity.
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