Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2020 Jun;35(3):348-350.
doi: 10.1007/s12250-020-00241-2. Epub 2020 Jun 19.

Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell

Pingping Yao  1 , Yachun Zhang  2 , Yisheng Sun  1 , Yulin Gu  2 , Fang Xu  1 , Bo Su  2 , Chen Chen  1 , Hangjing Lu  1 , Dehui Wang  2 , Zhangnv Yang  1 , Biao Niu  2 , Jiancai Chen  1 , Lixia Xie  2 , Lei Chen  2 , Yajing Zhang  2 , Hui Wang  2 , Yuying Zhao  2 , Yue Guo  2 , Juncheng Ruan  2 , Zhiyong Zhu  1 , Zhenfang Fu  3   4 , Dayong Tian  5 , Qi An  6 , Jianmin Jiang  7 , Hanping Zhu  8
Affiliations
Comment

Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell

Pingping Yao et al. Virol Sin. 2020 Jun.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Characteristics of SARS-CoV-2. A The cytopathic effect was observed in Vero cells infected with the isolated viruses at 5 dpi. B Nucleotide mutations of isolated viruses at passage 3. The SARS-CoV-2 infected-cells were homogenized with Trizol, and total RNA was extracted and was used for RT-PCR as described below. First-strand cDNA was synthesized with oligo(dT) primers, then the complete genome was segmented amplified and sequenced. Sequencing results were analyzed by SnapGene. C Phylogenetic tree was generated by Clustal Omega based on amino acid sequence of seven isolates and part of the available viruses. D Sequence results of 8#, from Passage 2 to Passage 4. E The multiple growth curve of isolated viruses. The virus growth curves were drawn based on the viral titers measured at each indicated time point. The growth curves were obtained by three independent experiments.
See this image and copyright information in PMC

Comment on

  • A pneumonia outbreak associated with a new coronavirus of probable bat origin.
    Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, Chen HD, Chen J, Luo Y, Guo H, Jiang RD, Liu MQ, Chen Y, Shen XR, Wang X, Zheng XS, Zhao K, Chen QJ, Deng F, Liu LL, Yan B, Zhan FX, Wang YY, Xiao GF, Shi ZL. Zhou P, et al. Nature. 2020 Mar;579(7798):270-273. doi: 10.1038/s41586-020-2012-7. Epub 2020 Feb 3. Nature. 2020. PMID: 32015507 Free PMC article.

References

    1. Kim JY, Ko JH, Kim Y, Kim YJ, Kim JM, Chung YS, Kim HM, Han MG, Kim SY, Chin BS. Viral load kinetics of SARS-CoV-2 infection in first two patients in Korea. J Korean Med Sci. 2020;35:e86. doi: 10.3346/jkms.2020.35.e86. - DOI - PMC - PubMed
    1. Kint J, Maier HJ, Jagt E. Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo. Methods Mol Biol. 2015;1282:89–98. doi: 10.1007/978-1-4939-2438-7_9. - DOI - PMC - PubMed
    1. Lai CC, Shih TP, Ko WC, Tang HJ, Hsueh PR. Severe acute respiratory syndrome coronavirus2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): the epidemic and the challenges. Int J Antimicrob Agents. 2020;55:105924. doi: 10.1016/j.ijantimicag.2020.105924. - DOI - PMC - PubMed
    1. Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395:565–574. doi: 10.1016/S0140-6736(20)30251-8. - DOI - PMC - PubMed
    1. Ren LL, Wang YM, Wu ZQ, Xiang ZC, Guo L, Xu T, et al. Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study. Chin. Med. J. (Engl.) 2020;133:1015–1024. doi: 10.1097/CM9.0000000000000722. - DOI - PMC - PubMed

LinkOut - more resources