doi: 10.1016/s0022-5193(88)80246-7.
A novel method for nucleic acid sequence determination
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- PMID: 3256722
- DOI: 10.1016/s0022-5193(88)80246-7
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A novel method for nucleic acid sequence determination
J Theor Biol.
.
Abstract
We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.
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