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. 2020 Oct;26(4):2469-2473.
doi: 10.1007/s12253-020-00818-y. Epub 2020 Jun 22.

Drastic Reduction of Turnaround Time After Implementation of a Fully Automated Assay for RAS-BRAF Mutations in Colorectal Cancer: A Pilot Prospective Study in Real-life Conditions

Stéphane Rossat  1 , Hervé Perrier  2 , Marine Lefevre  3 , Christophe Louvet  3 , Nathalie Le Berre  4 , Jérôme Chamois  5 , Maryline Dorel  6 , Daniel Vacque  7 , Angélique Guillaudeau  8 , Dominique Genet  9 , Evelyne Maillet  10 , Simon Triby  11 , Jean-Christophe Sabourin  12   13
Affiliations
Free article

Drastic Reduction of Turnaround Time After Implementation of a Fully Automated Assay for RAS-BRAF Mutations in Colorectal Cancer: A Pilot Prospective Study in Real-life Conditions

Stéphane Rossat et al. Pathol Oncol Res. 2020 Oct.
Free article

Abstract

In some situations, there is a need for rapid mutation tests for guiding clinical decisions and starting targeted therapies with minimal delays. In this study we evaluated the turnaround time before and after the implementation of a fully automated multiplex assay for KRAS and NRAS/BRAF mutation tests (Idylla™ platform, Biocartis) in metastatic colorectal cancer. The objective of this project was to compare the turnaround times in 2017-2018 with the fully automated multiplex assay to the 2016 results with previous methods. Centers with a number of tests for metastatic colorectal cancer > 100 yearly and a usual turnaround time ≥ 3 weeks for mutation detection were selected. Results of 505 KRAS tests and 369 NRAS/BRAF tests were transmitted by 10 centers. The mean turnaround time from test prescription to reception of results was reduced from 25.8 days in 2016 to 4.5 days in 2017-2018. In conclusion, this pilot project shows that the Idylla™ platform for testing KRAS and NRAS/BRAF mutations allows an optimized turnaround time from test prescription to reception of results.

Keywords: BRAF mutation; KRAS mutation; Metastatic colorectal cancer; Mutation analysis.

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