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. 2020 Dec;9(1):290-301.
doi: 10.1080/21623945.2020.1785083.

A new human adipocyte model with PTEN haploinsufficiency

Franziska Kässner  1 , Anna Kirstein  1 , Norman Händel  2 , Gordian L Schmid  3 , Kathrin Landgraf  1 , Antje Berthold  1 , Astrid Tannert  4 , Michael Schaefer  5 , Martin Wabitsch  6 , Wieland Kiess  1 , Antje Körner  1 , Antje Garten  1   7
Affiliations

A new human adipocyte model with PTEN haploinsufficiency

Franziska Kässner et al. Adipocyte. 2020 Dec.

Abstract

Few human cell strains are suitable and readily available as in vitro adipocyte models. We used resected lipoma tissue from a patient with germline phosphatase and tensin homolog (PTEN) haploinsufficiency to establish a preadipocyte cell strain termed LipPD1 and aimed to characterize cellular functions and signalling pathway alterations in comparison to the established adipocyte model Simpson-Golabi-Behmel-Syndrome (SGBS) and to primary stromal-vascular fraction cells. We found that both cellular life span and the capacity for adipocyte differentiation as well as adipocyte-specific functions were preserved in LipPD1 and comparable to SGBS adipocytes. Basal and growth factor-stimulated activation of the PI3 K/AKT signalling pathway was increased in LipPD1 preadipocytes, corresponding to reduced PTEN levels in comparison to SGBS cells. Altogether, LipPD1 cells are a novel primary cell model with a defined genetic lesion suitable for the study of adipocyte biology.

Keywords: 3D culture; AKT; Lipoma; SGBS; adipocyte differentiation; cellular life-span; phosphatases; phosphoinositide-3-kinase.

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Conflict of interest statement

All authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.
Characterization of lipoma tissue from a patient with phosphatase and tensin homolog (PTEN) hamartoma tumour syndrome
Figure 2.
Figure 2.
Growth and adipocyte differentiation of LipPD1 cells during long-term culture
Figure 3.
Figure 3.
Functional characteristics of LipPD1 compared to SGBS adipocytes
Figure 4.
Figure 4.
PI3 K signalling is constitutively activated in LipPD1 preadipocytes
See this image and copyright information in PMC

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