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. 2020 Apr;9(1):23-34.
doi: 10.22099/mbrc.2020.36031.1475.

Production of phenolic acids in hairy root cultures of medicinal plant Mentha spicata L. in response to elicitors

Shirin Yousefian  1 , Tahmineh Lohrasebi  1 , Mohsen Farhadpour  1 , Kamahldin Haghbeen  1
Affiliations

Production of phenolic acids in hairy root cultures of medicinal plant Mentha spicata L. in response to elicitors

Shirin Yousefian et al. Mol Biol Res Commun. 2020 Apr.

Abstract

In this study, hairy root induction in leaf and stem explants of Mentha spicata using various Agrobacterium rhizogenes strains was established for the first time. Although inoculation of explants by immersion method resulted in tissue necrosis, direct injection of explants by all examined strains (A13,R318,A4,GMI 9534 and ATCC15834) was effective. All different parts of the stem were susceptible to A. rhizogenes infection. However, the middle and lower internodes showed a higher rate of transformation. Among the different strains, the strain A13 exhibited the highest infection efficiency (almost 75% of the explants). A13 and R318-infected hairy roots showed the highest biomass production (close to 60 mg/flask), while infection with GMI 9534 produced the highest content of phenolic acids. Finally, the effect of phytohormone elicitation on hairy root growth and phenolic acid biosynthesis was investigated. A substantial increase in root growth and phenolic acids accumulation was obtained followed by 0.3 mg L-1 IBA and 100 µM MeJA treatment, respectively.

Keywords: Agrobacterium rhizogenes; Hairy root; IBA; MeJA; Mentha spicata; Phenolic acids.

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Figures

Figure 1
Figure 1
Comparative statistical positive responses of the leaf and stem explants of M. spicata to direct injection by A.rhizogenes
Figure 2
Figure 2
The percentage of hairy root induction in different parts of stem
Figure 3
Figure 3
(A) The percentage of hairy root induction in different strains of A. rhizogenes. (B) Growth rate of different strains of A. rhizogenes. Different letters on columns show significant difference between the strains
Figure 4
Figure 4
The content level of CGA (a), CIA (b), LAB (c), CA (d) and RA (e) in response to different A. rhizogenes strains. The comparison of five phenolic acids content among different strains (f). Different letters on columns show significant difference between the strains
Figure 5
Figure 5
Heatmap represents the changes in phenolic acids content in response to different A. rhizogenes strains and elicitation. The relative levels of each metabolite are indicated by the intensity of the blue color in which light blue and dark blue represent low and high concentrations of phenolic acids, respectively
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