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Observational Study
. 2021 Jun;52(2):625-633.
doi: 10.1007/s12029-019-00353-3.

Dysregulated Expression of Apoptosis-Associated Genes and MicroRNAs and Their Involvement in Gastric Carcinogenesis

Mayara Luciana Sallas  1 , Diana Zapparoli  1 , Mônica Pezenatto Dos Santos  1 , Jéssica Nunes Pereira  1 , Wilson Aparecido Orcini  1 , Rita Luiza Peruquetti  1 , Elizabeth Suchi Chen  2 , Marilia de Arruda Cardoso Smith  2 , Spencer Luiz Marques Payão  3 , Lucas Trevizani Rasmussen  4
Affiliations
Observational Study

Dysregulated Expression of Apoptosis-Associated Genes and MicroRNAs and Their Involvement in Gastric Carcinogenesis

Mayara Luciana Sallas et al. J Gastrointest Cancer. 2021 Jun.

Abstract

Purpose: Analyze the expression of caspase-9, Smac/DIABLO, XIAP, let-7a, and let-7b in patients with normal gastric tissue, chronic gastritis, and gastric adenocarcinoma.

Methods: The expression of caspase-9, Smac/DIABLO, XIAP, let-7a, and let-7b by qRT-PCR was analyzed in 158 samples from 53 patients with normal gastric mucosa, 86 with chronic gastritis, and 19 with gastric cancer.

Results: The comparison between the gastric cancer and the control group revealed a decreased expression of caspase-9 in gastric cancer tissues; considering the Helicobacter pylor presence, comparable results were revealed. Smac/DIABLO was increased in gastric cancer cells, while XIAP demonstrated no significant difference in the gene expression. The microRNA analysis revealed a decreased expression of let-7a and let-7b in samples positive to H. pylori infection and in gastric cancer group, regardless of the presence of the bacterium.

Conclusion: Our study provided some evidence of low activity of the intrinsic apoptosis pathway, as well as the influence of H. pylori on let-7a and let-7b expression.

Keywords: Caspase-9; Gastric cancer; Smac/DIABLO; XIAP; miR-let-7a; miR-let-7b.

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