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. 2020 Aug 7;56(61):8631-8634.
doi: 10.1039/d0cc02181d. Epub 2020 Jun 26.

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

Stefanie Brands  1 , Hannah U C Brass, Andreas S Klein, Jörg Pietruszka, Anna Joëlle Ruff, Ulrich Schwaneberg
Affiliations

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

Stefanie Brands et al. Chem Commun (Camb). .

Abstract

A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.

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