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Comparative Study
. 1988 Feb;32(2):175-9.
doi: 10.1128/AAC.32.2.175.

Detection of plasmid-mediated beta-lactamases with DNA probes

S Huovinen  1 , P Huovinén, G A Jacoby
Affiliations
Comparative Study

Detection of plasmid-mediated beta-lactamases with DNA probes

S Huovinen et al. Antimicrob Agents Chemother. 1988 Feb.

Abstract

beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical isolates making a variety of enzyme types. All strains producing a probe-type enzyme gave a positive hybridization reaction. Cross-hybridization was observed between TEM-1 and TEM-2 or TLE-1, between SHV-1 and SHV-2, between OXA-1 and OXA-4, between OXA-2 and OXA-3 (weak), between PSE-2 and OXA-6 or OXA-5 (weak), and among PSE-1, PSE-4, and CARB-3. With allowance for such cross-hybridization, only six strains gave false-positive reactions, and the procedure was 99% specific.

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References

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