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Review
. 2018 Dec 14;5(1):165.
doi: 10.18063/ijb.v5i1.165. eCollection 2019.

New microorganism isolation techniques with emphasis on laser printing

V S Cheptsov  1 , S I Tsypina  2 , N V Minaev  2 , V I Yusupov  2 , B N Chichkov  2   3
Affiliations
Review

New microorganism isolation techniques with emphasis on laser printing

V S Cheptsov et al. Int J Bioprint. .

Erratum in

  • ERRATUM.
    [No authors listed] [No authors listed] Int J Bioprint. 2020 Sep 17;6(4):309. doi: 10.18063/ijb.v6i4.309. eCollection 2020. Int J Bioprint. 2020. PMID: 33102924 Free PMC article.

Abstract

The study of biodiversity, growth, development, and metabolism of cultivated microorganisms is an integral part of modern microbiological, biotechnological, and medical research. Such studies require the development of new methods of isolation, cultivation, manipulation, and study of individual bacterial cells and their consortia. To this end, in recent years, there has been an active development of different isolation and three-dimensional cell positioning methods. In this review, the optical tweezers, surface heterogeneous functionalization, multiphoton lithography, microfluidic techniques, and laser printing are reviewed. Laser printing is considered as one of the most promising techniques and is discussed in detail.

Keywords: Laser printing; bacteria isolation; biodiversity; soil; unculturable microorganisms.

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Figures

Figure 1
Figure 1
The advantages of the RISL method over the standard ultraviolet photolithography. “Reprinted with permission from (Xu L, Robert L., Ouyang Q., Taddei F., Chen Y., Lindner A. B., Baigl D. Microcontact printing of living bacteria arrays with cellular resolution//Nano Lett. -2007. Vol. 7 - № 7. - P. 2068–2072). Copyright (2007) American Chemical Society.”
Figure 2
Figure 2
Gelatin-based micro-three-dimensional printing in the presence of bacteria. (Left) engineering polymicrobial communities (right) “Reprinted from (Connell J.L., Ritschdorff E.T., Whiteley M., Shear J.B. 3D printing of microscopic bacterial communities // Proc. Natl. Acad. Sci. - 2013. - Vol. 110 - № 46. - P. 18380–18385).”
Figure 3
Figure 3
Schematic sketch of the laser-assisted bioprinting.
Figure 4
Figure 4
Gel/soil microdroplets on an acceptor plate (A), soil microparticles distribution in microdroplets (B), and colonies as the result of microbial growth after gel/soil printing of gel/soil microdroplets onto agar plates (C) with E = 20 µJ.
Figure 5
Figure 5
Cultivated and identified groups of G+ and G− bacteria from the mollisol soil using the standard method and laser engineering of microbial systems technology.
Figure 6
Figure 6
A diagram illustrating the main differences between the laser engineering of microbial systems and standard method, leading to an increase in biodiversity in the isolation of microorganisms from soil. The numbers indicate microbes that, with the standard cultivation method: 1 - easy to flush out of their microenvironment, 2 - most actively multiply, 3 - separate from those with which they exist in symbiosis, and 4 - remain in the “sleeping” state.
See this image and copyright information in PMC

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