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. 2020 Aug;34(8):10623-10639.
doi: 10.1096/fj.202000088RR. Epub 2020 Jun 28.

The miR-140-5p/KLF9/KCNQ1 axis promotes the progression of renal cell carcinoma

Chenchen Huang  1   2 , Jianfa Li  1 , Xiaoting Zhang  3 , Tiefu Xiong  1 , Jing Ye  1 , Jing Yu  4 , Yaoting Gui  1   2
Affiliations

The miR-140-5p/KLF9/KCNQ1 axis promotes the progression of renal cell carcinoma

Chenchen Huang et al. FASEB J. 2020 Aug.

Erratum in

Abstract

Although renal cell carcinoma (RCC) is a common malignant urological cancer, its pathogenesis remains unclear. Previous studies have indicated that miR-140-5p acts as a tumor suppressor in various tumors, including bladder cancer, hepatocellular carcinoma, and gastric cancer, but its biological function in RCC remains unknown. In the present study, we found that miR-140-5p was upregulated in RCC tissues, whereas Krüppel-like factor 9 (KLF9) was downregulated and correlated inversely with miR-140-5p in RCC tissues. miR-140-5p promoted the proliferation, migration, and invasion of RCC cells in vitro, and knockdown of miR-140-5p significantly suppressed tumor growth and lung metastasis in nude mouse model of RCC. We also found that miR-140-5p significantly suppressed the expression of KLF9 by binding to the 3'-UTR of KLF9 mRNA and that KLF9, as a transcription factor, upregulates KCNQ1 (also called Kv 7.1 and Kv LQT1) expression by binding to the site (-841/-827) in the KCNQ1 promoter region in RCC cells. Moreover, forced expression of KCNQ1 decreased the growth and metastasis of RCC cells. These results suggest that the miR-140-5p/KLF9/KCNQ1 axis functions as a key signaling pathway in RCC progression and metastasis and represents a potential target of RCC therapies.

Keywords: KCNQ1; KLF9; miR-140-5p; proliferation/invasion/metastasis; renal cell carcinoma.

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Comment in

  • Uro-Science.
    Atala A. Atala A. J Urol. 2021 Apr;205(4):1228-1229. doi: 10.1097/JU.0000000000001605. Epub 2021 Jan 21. J Urol. 2021. PMID: 33472375 No abstract available.

References

REFERENCES

    1. Siegel RL, Miller KD, Jemal A. Cancer Statistics, 2017. CA Cancer J Clin. 2017;67:7-30.
    1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65:87-108.
    1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA Cancer J Clin. 2018;68:7-30.
    1. Barata PC, Rini BI. Treatment of renal cell carcinoma: current status and future directions. CA Cancer J Clin. 2017;67:507-524.
    1. Hollingsworth JM, Miller DC, Daignault S, Hollenbeck BK. Five-year survival after surgical treatment for kidney cancer: a population-based competing risk analysis. Cancer. 2007;109:1763-1768.

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