SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

doi:10.1007/s12250-020-00251-0

Comment

. 2020 Jun;35(3):355-358.

doi: 10.1007/s12250-020-00251-0. Epub 2020 Jun 29.

SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

Han Xia^{1

2}, Evans Atoni^{1

2}, Lu Zhao^{1

2}, Nanjie Ren^{1

2}, Doudou Huang¹, Rongjuan Pei^{1

2}, Zhen Chen¹, Jin Xiong¹, Raphael Nyaruaba^{1

2}, Shuqi Xiao¹, Bo Zhang^{1

2}, Zhiming Yuan^{3

4}

Affiliations

¹ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China. yzm@wh.iov.cn.
⁴ University of Chinese Academy of Sciences, Beijing, 100049, China. yzm@wh.iov.cn.

PMID: 32602045
PMCID: PMC7322373
DOI: 10.1007/s12250-020-00251-0

Comment

SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

Han Xia et al. Virol Sin. 2020 Jun.

. 2020 Jun;35(3):355-358.

doi: 10.1007/s12250-020-00251-0. Epub 2020 Jun 29.

Authors

Affiliations

¹ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China. yzm@wh.iov.cn.
⁴ University of Chinese Academy of Sciences, Beijing, 100049, China. yzm@wh.iov.cn.

PMID: 32602045
PMCID: PMC7322373
DOI: 10.1007/s12250-020-00251-0

No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interests.

Figures

**Fig. 1**
Replication of SARS-CoV-2 in different cell lines and field mosquitoes collected. A SARS-CoV-2 RNA copies detected in Vero E6 cells, C6/36 cells, Aag2 cells, and Sf9 cells evaluated using qRT-PCR at different time points after innoculation. Each cell line was repeated three times. B Indirect immunofluorescence assay (IFA) to detect the NP protein of SARS-CoV-2 in the four tested cell lines at 36 h post-inoculation. Bars, 100 µm. C Location of mosquito trap sites in the region of Wuhan City, China. Black dots stand for the 13 administrative districts of Wuhan, and Red triangles are the mosquito collection sites.

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References

1. Chan JF-W, Yuan S, Kok K-H, To KK-W, Chu H, Yang J, Xing F, Liu J, Yip CC-Y, Poon RW-S, Tsoi H-W, Lo SK-F, Chan K-H, Poon VK-M, Chan W-M, Ip JD, Cai J-P, Cheng VC-C, Chen H, Hui CK-M, Yuen K-Y. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. The Lancet. 2020;395:514–523. doi: 10.1016/S0140-6736(20)30154-9. - DOI - PMC - PubMed
1. Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R, Breitbart RE, Acton S. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res. 2000;87:E1–E9. doi: 10.1161/01.RES.87.5.e1. - DOI - PubMed
1. Ekbote U, Coates D, Isaac RE. A mosquito (Anopheles stephensi) angiotensin I-converting enzyme (ACE) is induced by a blood meal and accumulates in the developing ovary. FEBS Lett. 1999;455:219–222. doi: 10.1016/S0014-5793(99)00870-4. - DOI - PubMed
1. Franz AW, Kantor AM, Passarelli AL, Clem RJ. Tissue barriers to arbovirus infection in mosquitoes. Viruses. 2015;7:3741–3767. doi: 10.3390/v7072795. - DOI - PMC - PubMed
1. Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G, van Goor H. Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol. 2004;203:631–637. doi: 10.1002/path.1570. - DOI - PMC - PubMed

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[1] Chan JF-W, Yuan S, Kok K-H, To KK-W, Chu H, Yang J, Xing F, Liu J, Yip CC-Y, Poon RW-S, Tsoi H-W, Lo SK-F, Chan K-H, Poon VK-M, Chan W-M, Ip JD, Cai J-P, Cheng VC-C, Chen H, Hui CK-M, Yuen K-Y. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. The Lancet. 2020;395:514–523. doi: 10.1016/S0140-6736(20)30154-9. - DOI - PMC - PubMed

[2] Chan JF-W, Yuan S, Kok K-H, To KK-W, Chu H, Yang J, Xing F, Liu J, Yip CC-Y, Poon RW-S, Tsoi H-W, Lo SK-F, Chan K-H, Poon VK-M, Chan W-M, Ip JD, Cai J-P, Cheng VC-C, Chen H, Hui CK-M, Yuen K-Y. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. The Lancet. 2020;395:514–523. doi: 10.1016/S0140-6736(20)30154-9. - DOI - PMC - PubMed

[3] Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R, Breitbart RE, Acton S. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res. 2000;87:E1–E9. doi: 10.1161/01.RES.87.5.e1. - DOI - PubMed

[4] Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R, Breitbart RE, Acton S. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res. 2000;87:E1–E9. doi: 10.1161/01.RES.87.5.e1. - DOI - PubMed

[5] Ekbote U, Coates D, Isaac RE. A mosquito (Anopheles stephensi) angiotensin I-converting enzyme (ACE) is induced by a blood meal and accumulates in the developing ovary. FEBS Lett. 1999;455:219–222. doi: 10.1016/S0014-5793(99)00870-4. - DOI - PubMed

[6] Ekbote U, Coates D, Isaac RE. A mosquito (Anopheles stephensi) angiotensin I-converting enzyme (ACE) is induced by a blood meal and accumulates in the developing ovary. FEBS Lett. 1999;455:219–222. doi: 10.1016/S0014-5793(99)00870-4. - DOI - PubMed

[7] Franz AW, Kantor AM, Passarelli AL, Clem RJ. Tissue barriers to arbovirus infection in mosquitoes. Viruses. 2015;7:3741–3767. doi: 10.3390/v7072795. - DOI - PMC - PubMed

[8] Franz AW, Kantor AM, Passarelli AL, Clem RJ. Tissue barriers to arbovirus infection in mosquitoes. Viruses. 2015;7:3741–3767. doi: 10.3390/v7072795. - DOI - PMC - PubMed

[9] Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G, van Goor H. Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol. 2004;203:631–637. doi: 10.1002/path.1570. - DOI - PMC - PubMed

[10] Hamming I, Timens W, Bulthuis ML, Lely AT, Navis G, van Goor H. Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. J Pathol. 2004;203:631–637. doi: 10.1002/path.1570. - DOI - PMC - PubMed

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SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

Affiliations

SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

Authors

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Conflict of interest statement

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