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. 2020 Oct;42(10):e12771.
doi: 10.1111/pim.12771. Epub 2020 Aug 7.

Prospective study of serum and aqueous humour anti-Hsp70.1 IgG antibody levels in ocular toxoplasmosis

Christophe Chiquet  1 , Bernabé Chumpitazi  2 , Isabelle Vilgrain  3 , Anne Lesoin  1 , Helene Fricker-Hidalgo  2 , Marie-Pierre Brenier-Pinchart  2 , Caroline Vasseneix  4 , Olivier Savy  5 , Nelly Campolmi  6 , Philippe Gain  6 , Pierre Flori  7 , Florent Aptel  1 , Hervé Pelloux  2 , Laurence Bouillet  3   8
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Prospective study of serum and aqueous humour anti-Hsp70.1 IgG antibody levels in ocular toxoplasmosis

Christophe Chiquet et al. Parasite Immunol. 2020 Oct.

Abstract

Aims: We evaluate whether the serum and aqueous humour (AH) level of IgG anti-Hsp70.1 antibodies improved the biological diagnosis of ocular toxoplasmosis.

Methods and results: In this prospective cross-sectional and multicentre study, serum and AH were collected at the time of active uveitis. Anti-Hsp70.1-antibody levels were determined by ELISA. Patients with confirmed (Group A1, n = 21) or suspected ocular toxoplasmosis (group A2, n = 30) were enrolled, as well as a control group of patients with cataract (group B, n = 42). Serum IgG anti-Hsp70.1 antibody levels were not significantly different within the group of uveitis patients (A1, n = 21 vs A2, n = 30, P = .8) and were significantly associated with the affected retinal zone (P = .006) and with the size of the retinal lesion (P = .03). Serum anti-Hsp70.1 antibody level was positive in 10 out of the 18 patients of group A2. Significant anti-Hsp-70.1 antibody level in AH was reported in only three patients (3 eyes) with confirmed ocular toxoplasmosis.

Conclusion: While the level of IgG anti-Hsp-70.1 antibody in AH did not improve the laboratory diagnosis of ocular toxoplasmosis, its level in serum was of major significance for retinal damage diagnosis.

Keywords: Toxoplasma gondii; anti-Hsp70.1 antibody; diagnosis; ocular toxoplasmosis; uveitis.

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References

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