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. 2020 Jun 26;8(6):962.
doi: 10.3390/microorganisms8060962.

Antibacterial Activity of Four Plant Extracts Extracted from Traditional Chinese Medicinal Plants against Listeria monocytogenes, Escherichia coli, and Salmonella enterica subsp. enterica serovar Enteritidis

R L McMurray  1 , M E E Ball  2 , M M Tunney  3 , N Corcionivoschi  4 , C Situ  5
Affiliations

Antibacterial Activity of Four Plant Extracts Extracted from Traditional Chinese Medicinal Plants against Listeria monocytogenes, Escherichia coli, and Salmonella enterica subsp. enterica serovar Enteritidis

R L McMurray et al. Microorganisms. .

Abstract

The worldwide ethnobotanical use of four investigated plants indicates antibacterial properties. The aim of this study was to screen and determine significant antibacterial activity of four plant extracts in vitro and in a poultry digest model. Using broth microdilution, the concentrations at which four plant extracts inhibited Listeria monocytogenes, Salmonella enteritidis, and Escherichia coli over 24 hours was determined. Agrimonia pilosa Ledeb, Iris domestica (L.) Goldblatt and Mabb, Anemone chinensis Bunge, and Smilax glabra Roxb all exhibited a minimum inhibitory concentration (MIC) of 62.5mg/L and a minimum bactericidal concentration (MBC) of 500mg/L against one pathogen. A. pilosa Ledeb was the most effective against L. monocytogenes and E. coli with the exception of S. enteritidis, for which A. chinensis Bunge was the most effective. Time-kills of A. pilosa Ledeb and A. chinensis Bunge against L. monocytogenes, E. coli and S. enteritidis incubated in poultry cecum were used to determine bactericidal activity of these plant extracts. A. chinensis Bunge, significantly reduced S. enteritidis by ≥ 99.99% within 6 hours. A. pilosa Ledeb exhibited effective significant bactericidal activity within 4 hours against L. monocytogenes and E. coli. This paper highlights the potential of these plant extracts to control pathogens commonly found in the poultry gastrointestinal tract.

Keywords: antibiotic resistance; antimicrobial susceptibility; broiler; broth microdilution; chicken; digest; in vitro model; pathogens; plant extracts; time–kill assay.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Total viable count of endogenous and inoculated Listeria monocytogenes QA1018, LS12519, and CP102 mixture in the presence of Agrimonia pilosa Ledeb.
Figure 2
Figure 2
Total viable count of endogenous and inoculated E. coli UM004, UM011, and UM012 mixture in the presence of Agrimonia pilosa Ledeb.
Figure 3
Figure 3
Total viable count of endogenous and inoculated Salmonella enteritidis QA0419, LE103, and 1F6144 mixture in the presence of Anemone chinensis Bunge.
See this image and copyright information in PMC

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