Secretory immune responses in ageing rats. II. Phenotype distribution of lymphocytes in secretory and lymphoid tissues

Abstract

The distribution of lymphocyte phenotypes was examined in various tissues from weanling (21-35 days), adult (3-4 months), mid-life (10-12 months) and senescent (18-20 months) rats. Lymphoid tissues included peripheral blood, spleen, cervical, mesenteric and inguinal lymph nodes. Tissues associated with secretory immune responses were also examined, including submandibular and parotid salivary glands, extraorbital lacrimal glands and Peyer's patches. IgA, IgG and IgM B cells were determined by surface Ig staining. Total T cells (W3/13), T helper/inducer (Th) (W3/25), T suppressor/cytotoxic (Ts) (OX8) and immature T cells (Thy 1.1; OX7) were also evaluated. IgG B cells were significantly decreased in lymphoid tissues from the senescent rats, while the weanling group exhibited decreased levels of all three B-cell isotypes compared to adult animals. IgA B cells were significantly decreased in the secretory tissues of the senescent rats, while IgM B cells were increased in both the weanling and senescent groups. Total T-cell percentages were unaffected by ageing in any of the tissues. The only consistent alteration in the lymphoid tissues was a decrease in Thy 1.1-positive cells in the older groups compared to the weanling group. A decreased Th cell percentage was demonstrated in the salivary and lacrimal glands of the weanling and senescent groups. Decreases in Th/Ts ratios, as well as decreased numbers of plasma cell precursors in the secretory tissues of the aged rats, suggests that alterations in normal secretory immune responses may be expected to accompany the ageing process.