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. 2020:2164:129-143.
doi: 10.1007/978-1-0716-0704-6_14.

A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

Sijia Yu  1   2 , Santiago Vernia  3   4
Affiliations

A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection

Sijia Yu et al. Methods Mol Biol. 2020.

Abstract

Transgenic mouse are reliable, convenient models for studying human hepatocellular carcinoma (HCC). The development of a synthetically engineered Sleeping Beauty (SB) transposon system further enables the viral-free, efficient delivery of desired oncogenes to mouse tissues. Here, we describe an SB transposon-based approach to induce HCC in mice by expressing a hyperactive form of N-RAS, N-RASG12V, while silencing the endogenous Trp53 gene via hydrodynamic tail vein injection, a method to rapidly deliver naked plasmids to mouse liver.

Keywords: Hepatocellular carcinoma; Hydrodynamic tail vein injection; Mouse tumor model; N-RAS; Sleeping Beauty; p53.

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