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. 2020:2156:99-117.
doi: 10.1007/978-1-0716-0660-5_9.

Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Seizo Fujikawa  1 , Keita Endoh  2
Affiliations

Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Seizo Fujikawa et al. Methods Mol Biol. 2020.

Abstract

A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, the essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.

Keywords: Cooling rate; Cryo-fixation; Cryo-scanning electron microscope (cryo-SEM); Extracellular freezing; Ice crystal; Intracellular freezing; Recrystallization.

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