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. 2020 Dec;27(1):953-963.
doi: 10.1080/10717544.2020.1775722.

Biological investigations on therapeutic effect of chitosan encapsulated nano resveratrol against gestational diabetes mellitus rats induced by streptozotocin

Shengye Du  1 , Yan Lv  2 , Na Li  3 , Xianxia Huang  1 , Xuemei Liu  1 , Hui Li  1 , Chao Wang  1 , Yi-Fang Jia  4
Affiliations

Biological investigations on therapeutic effect of chitosan encapsulated nano resveratrol against gestational diabetes mellitus rats induced by streptozotocin

Shengye Du et al. Drug Deliv. 2020 Dec.

Abstract

The chitosan encapsulation with bioactive compounds (resveratrol) is a significant method that can be used to raise the stability and effectiveness of substances in gestational diabetes management. In this study, the resveratrol-zinc oxide complex is encapsulated with chitosan (CS-ZnO-RS). The synthesized CS-ZnO-RS could be used to deliver the resveratrol with minimized side effects and also improved bioavailability. CS-ZnO-RS were characterized by various techniques such as particle size analyzer, DSC, FT-IR, TEM, SEM, and AFM. The electron microscopic and particle analyzer confirmed that the synthesized CS-ZnO-RS were monodispersed, spherical and its average size was 38 nm. The drug-releasing profile showed that 95% of RS is released from CS-ZnO-RS within 24 h. In vitro studies confirmed that α-glucosidase and α-amylase inhibitory activities were closely related to the concentration of CS-ZnO-RS. The highest inhibition of α-glucosidase (77.32%) and α-amylase (78.4%) was observed at 500 μg/mL. Furthermore, the treatment of CS-ZnO-RS significantly decreased the blood glucose levels in gestational diabetes mellitus induced rats and maintained the lipid content toward the normal rats. In addition, the CS-ZnO-RS reduced the level of inflammation factors (IL-6 and MCP-1) and endoplasmic reticulum stress (GRP78, p-IRE1α, p-eIF2α, and p-PERK).

Keywords: Gestational diabetes mellitus; chitosan; endoplasmic reticulum stress; resveratrol; streptozotocin.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

None
Graphical abstract
Figure 1.
Figure 1.
Microscopic characterization of CS–ZnO–RS; (A, A1) AFM, (B) TEM (inserted SAED), (C) SEM, (D) particle size analysis, and (E) zeta potential results of prepared CS–ZnO–RS nanoformulation.
Figure 2.
Figure 2.
(A) DSC and (B) FTIR spectrum (a, CS; b, RS; c, CS–ZnO–RS) of CS–ZnO–RS.
Figure 3.
Figure 3.
In vitro cumulative release of resveratrol CS–ZnO–RS nanoparticles.
Figure 4.
Figure 4.
In vitro examination of antidiabetic activity using α-glucosidase and α-amylase.
Figure 5.
Figure 5.
Fixation of optimum dosage level in Wister albino rats.
Figure 6.
Figure 6.
The homeostasis model valuation for insulin resistance (A) and HOMA for β-cell (B) in GDM and CS–ZnO–RS treated rats.
Figure 7.
Figure 7.
Evaluation of lipid profile in GDM and CS–ZnO–RS treated rats.
Figure 8.
Figure 8.
Assessment of antioxidant activities in in GDM and CS–ZnO–RS treated rats.
Figure 9.
Figure 9.
The mRNA expressions of IL-6 and MCP-1 in control and experimental rats using RT-PCR analysis.
Figure 10.
Figure 10.
The protein expression of endoplasmic reticulum stress-related factors (GRP78, p-IRE1α, and p-eIF2α) by western blot method.
Figure 11.
Figure 11.
The histopathological examination of control and experimental rat liver; (A, A1) control, (B, B1) diabetic mellitus rats (C, C1) diabetic rats treated with CS–ZnO–RS in different microscopic magnifications (×40 and ×10).
See this image and copyright information in PMC

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