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Case Reports
. 2020 Jun;25(25):2000929.
doi: 10.2807/1560-7917.ES.2020.25.25.2000929.

An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020

Stefan Monecke  1   2   3   4 , Elisabeth König  4   5 , Megan R Earls  4   6 , Eva Leitner  5 , Elke Müller  1   3 , Gabriel E Wagner  5 , David M Poitz  7 , Lutz Jatzwauk  8 , Teodora Vremerǎ  9 , Olivia S Dorneanu  9 , Alexandra Simbeck  10 , Andreas Ambrosch  11 , Ines Zollner-Schwetz  12 , Robert Krause  12 , Werner Ruppitsch  13 , Wulf Schneider-Brachert  10 , David C Coleman  6 , Ivo Steinmetz  5 , Ralf Ehricht  1   14   3
Affiliations
Case Reports

An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020

Stefan Monecke et al. Euro Surveill. 2020 Jun.

Abstract

We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.

Keywords: BD MAX, next generation sequencing; CC1-MRSA-IV; Cepheid GenXpert; Staphylococci; Staphylococcus aureus; commercial PCR.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure
Figure
SCCmec elements in the CC1 reference sequence MW2, the European CC1-MRSA-IV isolate Iasi-95037and the Staphylococcus epidermidis isolate P11PPP12
See this image and copyright information in PMC

References

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